IGEM:MIT/2006/Notebook/2007-1-24: Difference between revisions
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==Plan== | ==Plan== | ||
1. Since 600A looks to have a mixed sequencing result, will transform the miniprep before 10 AM- DONE and make LCs at night | 1. Since 600A looks to have a mixed sequencing result, will transform the miniprep before 10 AM- DONE and make LCs at night- DONE | ||
2. Will religate and retransform parts to make J45800 before 10 AM- DONE and make LCs at night | 2. Will religate and retransform parts to make J45800 before 10 AM- DONE and make LCs at night- NOT DONE (NONE GREW UP, PUT BACK IN WARM ROOM) | ||
3. Will make serial dilutions of methyl salicylate in culture, make samples of J45700 cultures, and make a negative control culture- DONE and test them all with the GC | 3. Will make serial dilutions of methyl salicylate in culture, make samples of J45700 cultures, and make a negative control culture- DONE and test them all with the GC- DONE | ||
4. Will discuss with Lily a proper protocol for extracting isoamyl acetate from culture- DONE | 4. Will discuss with Lily a proper protocol for extracting isoamyl acetate from culture- DONE | ||
5. | 5. Will read papers that Lily sent on isolating metabolites and derivitizing compounds- DONE | ||
6. Will make LCs of the proper osmY and control structures to run the plate reader experiment tomorrow- DONE | 6. Will make LCs of the proper osmY and control structures to run the plate reader experiment tomorrow- DONE | ||
7. Clean old tubes- DONE | |||
8. Add J45997 and J45998 to the Registry- DONE | |||
Latest revision as of 09:55, 25 January 2007
Plan
1. Since 600A looks to have a mixed sequencing result, will transform the miniprep before 10 AM- DONE and make LCs at night- DONE
2. Will religate and retransform parts to make J45800 before 10 AM- DONE and make LCs at night- NOT DONE (NONE GREW UP, PUT BACK IN WARM ROOM)
3. Will make serial dilutions of methyl salicylate in culture, make samples of J45700 cultures, and make a negative control culture- DONE and test them all with the GC- DONE
4. Will discuss with Lily a proper protocol for extracting isoamyl acetate from culture- DONE
5. Will read papers that Lily sent on isolating metabolites and derivitizing compounds- DONE
6. Will make LCs of the proper osmY and control structures to run the plate reader experiment tomorrow- DONE
7. Clean old tubes- DONE
8. Add J45997 and J45998 to the Registry- DONE
