IGEM:MIT/2006/Notebook/2007-1-25: Difference between revisions

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4. Religate and retransform J45170.J45320
4. Religate and retransform J45170.J45320


5. Smell the J45250.J45400 cultures and pick the best ones (wait until 2 PM)
5. Smell the J45250.J45400 cultures and pick the best ones (wait until 2 PM)- DONE


6. Miniprep and sequence the best J45250.J45400 cultures
6. Miniprep and sequence the best J45250.J45400 cultures

Revision as of 12:10, 25 January 2007

Plan

1. Run the osmY and control samples on the plate reader- DONE

2. Look over more literature on extracting isoamyl acetate and contact Lily about that and picking up the GC data- DONE (think I might have found how to extract isoamyl acetate)

3. Pick up the GC data- GC DATA DID NOT SHOW METHYL SALICYLATE!!! NO!!! WE WILL CONSIDER GOING BACK TO THE NONPOLAR COLUMN.

4. Religate and retransform J45170.J45320

5. Smell the J45250.J45400 cultures and pick the best ones (wait until 2 PM)- DONE

6. Miniprep and sequence the best J45250.J45400 cultures