IGEM:MIT/2006/Notebook/2007-1-25

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Revision as of 16:01, 25 January 2007 by Stephen (talk | contribs) (→‎Plan)
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Plan

1. Run the osmY and control samples on the plate reader- DONE

2. Look over more literature on extracting isoamyl acetate and contact Lily about that and picking up the GC data- DONE (will extract with pentane/ether mix)

3. Pick up the GC data- GC DATA DID NOT SHOW METHYL SALICYLATE!!! NO!!! WE WILL CONSIDER GOING BACK TO THE NONPOLAR COLUMN.

4. Religate and retransform J45170.J45320- DONE

5. Smell the J45250.J45400 cultures and pick the best ones (wait until 2 PM)- DONE

6. Miniprep- DONE and sequence the best J45250.J45400 cultures- DONE

7. Redilute isoamyl acetate producing cultures in case we are ready to use the GC tomorrow- DONE