IGEM:MIT/2006/Notebook/2007-1-27

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5. Religate and transform 170.320 mix  
5. Religate and transform 170.320 mix  
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*Stephen - I put cells in warm room at 4:25...so plate after 5:25 I guess? thanks! -Veena
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*Stephen - I put cells in warm room at 4:25...so plate after 5:25 I guess? I left plates out on the bench by the hot water bath. thanks! -Veena
6. Run 170 cut on gel
6. Run 170 cut on gel

Revision as of 17:04, 27 January 2007

Plan

1. Dilute the osmY and control structures to 1:250 in 5 mL culture and grow up for 1 hr- DONE

2. Run the osmY and control structures on the plate reader- DONE

3. Make LCs of the three 170.320 colonies- DONE

4. Redigest 170- DONE

5. Religate and transform 170.320 mix

  • Stephen - I put cells in warm room at 4:25...so plate after 5:25 I guess? I left plates out on the bench by the hot water bath. thanks! -Veena

6. Run 170 cut on gel

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