IGEM:MIT/2006/Notebook/2007-6-15: Difference between revisions
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*Note- always do a 1:100 dilution of the 1000 ug/mL stock solution of PCNB into methyl salicylate samples. | *Note- always do a 1:100 dilution of the 1000 ug/mL stock solution of PCNB into methyl salicylate samples. | ||
=Ligations= | =Ligations & Transformations= | ||
Did ligations of J45181 to J45320 and J45250 to J45400 in many various ways (see ligations sheet for details)- DONE | Did ligations of J45181 to J45320 and J45250 to J45400 in many various ways (see ligations sheet for details) and transformed them into top 10 competent cells- DONE | ||
Latest revision as of 15:01, 15 June 2007
Time Course of J45181
Ran GC/MS on all of the samples- DONE
- Note- always do a 1:100 dilution of the 1000 ug/mL stock solution of PCNB into methyl salicylate samples.
Ligations & Transformations
Did ligations of J45181 to J45320 and J45250 to J45400 in many various ways (see ligations sheet for details) and transformed them into top 10 competent cells- DONE
