IGEM:MIT/2006/Notebook/2007-6-19: Difference between revisions
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6. Set up reservation for GC/MS- DONE (3:00) | 6. Set up reservation for GC/MS- DONE (3:00) | ||
7. Do GC/MS for samples- | 7. Do GC/MS for samples- DONE (got approximately 1:1 ratio between conc. in media and conc. in sample, although .1 ppm and 5 ppm did not show any methyl salicylate found) | ||
8. Edit introduction from yesterday- DONE (to some degree) | 8. Edit introduction from yesterday- DONE (to some degree) | ||
9. Check sequencing results- some look good (will look with Barry to confirm | 9. Check sequencing results- some look good (will look with Barry to confirm)- DONE (800D1, 600D1, and 600D2 LINE UP!!! YEH!!! Small point mutations will investigate tomorrow) | ||
10. Make two 25 mL cultures (J45120 + salicylic acid and J45200 + isoamyl alcohol) for Drew- DONE | 10. Make two 25 mL cultures (J45120 + salicylic acid and J45200 + isoamyl alcohol) for Drew- DONE | ||
Latest revision as of 18:19, 19 June 2007
Things to do Today
1. Take the culture from last night out- DONE
2. Take OD600s of the culture- DONE (all 2.00)
3. Spike culture with methyl salicylate- DONE (.1 ppm, 1 ppm, 5 ppm, 10 ppm, 25 ppm, and negative control)
4. Extract samples from the culture- DONE
5. Repeat to 1) for next culture- DONE FOR ALL
6. Set up reservation for GC/MS- DONE (3:00)
7. Do GC/MS for samples- DONE (got approximately 1:1 ratio between conc. in media and conc. in sample, although .1 ppm and 5 ppm did not show any methyl salicylate found)
8. Edit introduction from yesterday- DONE (to some degree)
9. Check sequencing results- some look good (will look with Barry to confirm)- DONE (800D1, 600D1, and 600D2 LINE UP!!! YEH!!! Small point mutations will investigate tomorrow)
10. Make two 25 mL cultures (J45120 + salicylic acid and J45200 + isoamyl alcohol) for Drew- DONE
