IGEM:MIT/2006/Notebook/2007-6-23: Difference between revisions
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(New page: '''Results from extraction experiment yesterday''' One extraction was 300% efficient somehow, which could have been a result of it being 100 ppm concentration (a relatively high concentra...) |
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One extraction was 300% efficient somehow, which could have been a result of it being 100 ppm concentration (a relatively high concentration. For fear that I would break the machine at 200 ppm, I did not run that sample. The rest of the samples were pretty consistently at around 30%. This is very frustrating. I will repeat the experiment again some time. | One extraction was 300% efficient somehow, which could have been a result of it being 100 ppm concentration (a relatively high concentration. For fear that I would break the machine at 200 ppm, I did not run that sample. The rest of the samples were pretty consistently at around 30%. This is very frustrating. I will repeat the experiment again some time. | ||
=Things to Do Today | =Things to Do Today= | ||
1. Graph results from plate reader | 1. Graph results from plate reader- DONE (R0040.E0840 trumps the experimental groups, will repeat with slightly different protocol | ||
2. Reinvite Dr. Knight to look at the google doc | 2. Reinvite Dr. Knight to look at the google doc- DONE | ||
3. Contact Sauer lab about C18 column chromatography | 3. Contact Sauer lab about C18 column chromatography | ||
4. Contact Prather about what I found regarding chorismate (and cc Jason) | 4. Contact Prather about what I found regarding chorismate (and cc Jason) | ||
Revision as of 15:42, 23 June 2007
Results from extraction experiment yesterday
One extraction was 300% efficient somehow, which could have been a result of it being 100 ppm concentration (a relatively high concentration. For fear that I would break the machine at 200 ppm, I did not run that sample. The rest of the samples were pretty consistently at around 30%. This is very frustrating. I will repeat the experiment again some time.
Things to Do Today
1. Graph results from plate reader- DONE (R0040.E0840 trumps the experimental groups, will repeat with slightly different protocol
2. Reinvite Dr. Knight to look at the google doc- DONE
3. Contact Sauer lab about C18 column chromatography
4. Contact Prather about what I found regarding chorismate (and cc Jason)
