Things to Do Today

1. Get streaked plates out of the warm room- DONE (all look good except J45120 & J45250 (no isolated colonies))

2. Make LCs of J45995, J45996, R0040.E0840, and B0015 for plate reader experiment tomorrow- DONE

3. Reserve plate reader for tomorrow- DONE

4. Research leucine pathway

Use of plasmids: http://aem.asm.org/cgi/reprint/63/12/4651?view=long&pmid=9406383, http://www.freepatentsonline.com/20040091980.html, It has now been found, surprisingly, that isolation of the ilvE gene present in E. coli ATCC 11303, and its cloning onto a multicopy plasmid, results, after transformation of the starting strain with this plasmid, in an increase of at least 3- to 5-fold in the yield of the particular amino acid- http://www.freepatentsonline.com/5120654.html

5. Email leucine calculations to Endy/Knight/grad students- DONE

6. Heard back from Yale stock center. Need to clarify what I heard on the phone in my conversation with Tribe. Send Tribe an email- DONE

7. Order a chorismate-overproducing strain from Yale

8. Restreak J45120 & J45250

9. HPLC isoamyl acetate with Andreas at neutral pH- DONE (DID NOT WORK, TOO MANY PEAKS, MUST LOOK INTO GC/MS OPTIONS)

10. Found another GC in the geobiology lab. Contact their lab technician inquiring about using the GC- DONE

IGEM:MIT/2006/Notebook/2007-6-29