IGEM:MIT/2006/Notebook/2007-7-12
Things to Do Today
1. Write back Alex about mechanics of construction a calibration curve, internal standard, chromatograph data, etc.- DONE (awaiting reply to a few questions)
2. Call back Weston chemicals about ordering alpha-ketoisocaproate- DONE (awaiting reply)
3. Unfortunately, cannot do time course of J45181 due to the fact that there are not enough flasks
5. Bleach cultures from yesterday- DONE
6. Look at various flasks online to see if you can find a match with those that we have here- FOUND ONE (BUT TAKES LONG TO SHIP, WILL BE HERE LATE IN THE MONTH)
7. Email biostuff about obtaining more of the flasks- DONE
8. Draw up week-long plan for device characterization- DONE
9. Research the two conferences Drew told us about online- DONE
10. Get back to Drew on what he thinks is the better option- DONE
11. Make streptomycin plates for aroF- aroG- aroH- strain- DONE
12. aroF- aroG- aroH- strain in, grow it up on 2 streptomycin plates tonight- DONE
13. Make LCs of J45200 and J45250- DONE
14. Send thank you note to Berlyn/Wertz for aroF- aroG- aroH- strain- DONE
15. Send an email to grad students/Knight/Endy about meeting tomorrow- DONE
Week-long Plan
Friday- Time Course of J45181
4:15 AM set up big culture
4:40 AM split big culture
4:45 AM 0-hr time point
8:45 AM 4-hr time point- add SA
9:45 AM 5-hr time point- add SA, GC extract
10:45 AM 6-hr time point- add SA, GC extract
11:45 AM 7-hr time point- add SA, GC extract
12:45 PM 8-hr time point- add SA, GC extract
1:45 PM 9-hr time point- add SA, GC extract
2:45 PM 10-hr time point- add SA, GC extract
3:45 PM 11-hr time point- add SA, GC extract
4:45 PM 12-hr time point- add SA, GC extract
5:45 PM 13-hr time point- GC extract
Saturday- GC of J45181 samples
Monday- Time Course of J45120
Tuesday- GC of J45120 samples
Wednesday- growth curve of J45200
Thursday- growth curve of J45250