IGEM:MIT/2006/Notebook/2007-7-13: Difference between revisions
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8. Reply to Linda from Yale to make sure that she was referring to the IlvE- strain coming in the mail | 8. Reply to Linda from Yale to make sure that she was referring to the IlvE- strain coming in the mail | ||
=Updates= | |||
1. AB3257 (aroF- aroG- aroH-) did not grow up. | |||
2. Linda from Yale said that our strain is coming in today's mail (I am pretty sure she means the IlvE- strain) | |||
=Growth Curve Construction for J45200 and J45250= | =Growth Curve Construction for J45200 and J45250= |
Revision as of 06:56, 13 July 2007
Things to Do Today
1. Reply to Alex's email, making sure that you understood him right about what internal and external standards to order/interpretation of the data- DONE (awaiting reply)
2. Ask Li Li about internal and external standards to make sure that PCNB should not be added before GC extraction- DONE (awaiting reply)
3. Order hexyl acetate and octyl acetate as internal and external standards for isoamyl acetate samples
4. Contact Weston chemicals about alpha-ketoisocaproate (try to do by email this time)
5. Prep for 1:10 meeting on precursor biosynthesis
6. Construct growth curves for J45200 and J45250- WORKING ON IT
7. Restreak AB3257 (aroF- aroG- aroH- strain)
8. Reply to Linda from Yale to make sure that she was referring to the IlvE- strain coming in the mail
Updates
1. AB3257 (aroF- aroG- aroH-) did not grow up.
2. Linda from Yale said that our strain is coming in today's mail (I am pretty sure she means the IlvE- strain)
Growth Curve Construction for J45200 and J45250
J45200
Hour----------Culture 1---------Culture 2---------Culture 3---------Average
3---------------0.02---------------0.02---------------0.02----------------0.020
J45250
Hour----------Culture 1---------Culture 2---------Culture 3---------Average
3---------------0.06---------------0.06---------------0.08----------------0.067