IGEM:MIT/2006/Notebook/2007-7-13

Things to Do Today

1. Reply to Alex's email, making sure that you understood him right about what internal and external standards to order/interpretation of the data- DONE (I had it right. Get to ordering the chemicals)

2. Ask Li Li about internal and external standards to make sure that PCNB should not be added before GC extraction- DONE (awaiting reply)

3. Order hexyl acetate and octyl acetate as internal and external standards for isoamyl acetate samples- DONE

4. Contact Westco chemicals about alpha-ketoisocaproate (try to do by email this time)- DONE (awaiting reply)

5. Prep for 1:10 meeting on precursor biosynthesis- DONE

6. Construct growth curves for J45200 and J45250- WORKING ON IT

7. Restreak AB3257 (aroF- aroG- aroH- strain)- DONE

8. Reply to Linda from Yale to make sure that she was referring to the IlvE- strain coming in the mail- DONE (IlvE- is the one!)

9. Ask dishwashers about getting the baffled flasks cleaned for Monday morning so I can start the time courses on time- DONE (sweet, they're going to autoclave them first thing for me)

10. Ask Reshma about specifics of protein gel set for tomorrow- DONE (ready to go)

11. Set up cultures for tomorrow's protein gel- DONE

12, Ask Tom/Drew about making the appropriate media for overproducing chorismate experiment discussed at the meeting- DONE (reviewing Drew's response)

13. Get rid of large boxes- DONE

14. Set up a meeting for the beginning of next week on banana precursor biosynthesis/device characterization- DONE

Updates

1. AB3257 (aroF- aroG- aroH-) did not grow up.

2. Linda from Yale said that our strain is coming in today's mail (I am pretty sure she means the IlvE- strain)- SHE DID!

Growth Curve Construction for J45200 and J45250

J45200

Hour----------Culture 1---------Culture 2---------Culture 3---------Average

3---------------0.02---------------0.02---------------0.02----------------0.020

4---------------0.08---------------0.08---------------0.08----------------0.080

5---------------0.32---------------0.30---------------0.30----------------0.307

6---------------0.80---------------0.72---------------0.74----------------0.753

7---------------1.34---------------1.32---------------1.32----------------1.327

8---------------2.16---------------2.20---------------2.28----------------2.213

9---------------2.58---------------2.66---------------2.60----------------2.613

10--------------3.12---------------3.09---------------2.88----------------3.030

11--------------3.16---------------3.16---------------3.10----------------3.140

12--------------2.84---------------3.14---------------3.08----------------3.020

13--------------3.14---------------3.26---------------3.22----------------3.207

J45250

Hour----------Culture 1---------Culture 2---------Culture 3---------Average

3---------------0.06---------------0.06---------------0.08----------------0.067

4---------------0.26---------------0.28---------------0.28----------------0.267

5---------------0.78---------------0.76---------------0.76----------------0.767

6---------------1.68---------------1.68---------------1.66----------------1.673

7---------------2.42---------------2.42---------------2.44----------------2.427

8---------------2.80---------------2.86---------------2.96----------------2.873

9---------------3.06---------------3.10---------------3.22----------------3.127

10--------------3.24---------------3.22---------------3.30----------------3.253

11--------------3.26---------------3.26---------------3.26----------------3.260

12--------------3.22---------------3.12---------------3.08----------------3.140

13--------------3.24---------------3.22---------------3.20----------------3.220