IGEM:MIT/2006/Notebook/2007-7-18

Update

IlvE- strain came from Yale (the strain that will overproduce leucine when tyrB is overexpressed). Also, the leucine-overproducing plasmid that when transformed overproduces leucine in cells grown on media supplemented with ammonium formate.

To Do Today

1. Run time courses for J45120 and J45181 (n:53 (J45120) and n+1:13 (J45181) for n=12 through n=8 for addition of SA, for n=1 through n=9 for GC extraction)

2. Add known methyl salicylate to random cells and GC extract- DONE

3. Run the methyl salicylate samples on the GC for recalibration- DONE

4. Contact Li Li about good time to meet about recalibration- DONE

5. Smell cultures grown on Tom media- growing up very, very slowly or probably not at all- contact Tom- DONE

6. GC extract samples grown on Tom media- wait until they grow up more tomorrow

7. Take out AB3257, make a glycerol stock of it- DONE

8. Bleach bottles from last night- DONE

9. Write thank you notes to Yale Center and Dr. Esaki- DONE

10. Plate the IlvE- strain- DONE

11. Make LCs of J45181 and J45120 for next week- DONE

OD600s

Hour---------J45120---------J45181

5--------------0.14-------------0.24

6--------------0.42-------------0.50

7--------------0.84-------------0.92

8--------------1.38-------------1.46

9--------------1.84-------------1.82

10------------2.20-------------2.30

11------------2.26-------------2.20

12------------2.40-------------2.38

13------------2.48-------------2.52