IGEM:MIT/2006/Notebook/2007-7-18
Update
IlvE- strain came from Yale (the strain that will overproduce leucine when tyrB is overexpressed). Also, the leucine-overproducing plasmid that when transformed overproduces leucine in cells grown on media supplemented with ammonium formate.
To Do Today
1. Run time courses for J45120 and J45181 (n:53 (J45120) and n+1:13 (J45181) for n=12 through n=8 for addition of SA, for n=1 through n=9 for GC extraction)
2. Add known methyl salicylate to random cells and GC extract- DONE
3. Run the methyl salicylate samples on the GC for recalibration- DONE
4. Contact Li Li about good time to meet about recalibration- DONE
5. Smell cultures grown on Tom media- growing up very, very slowly or probably not at all- contact Tom- DONE
6. GC extract samples grown on Tom media- wait until they grow up more tomorrow
7. Take out AB3257, make a glycerol stock of it- DONE
8. Bleach bottles from last night- DONE
9. Write thank you notes to Yale Center and Dr. Esaki- DONE
10. Plate the IlvE- strain- DONE
11. Make LCs of J45181 and J45120 for next week- DONE
OD600s
Hour---------J45120---------J45181
5--------------0.14-------------0.24
6--------------0.42-------------0.50
7--------------0.84-------------0.92
8--------------1.38-------------1.46
9--------------1.84-------------1.82
10------------2.20-------------2.30
11------------2.26-------------2.20
12------------2.40-------------2.38
13------------2.48-------------2.52