IGEM:MIT/2006/Notebook/2007-7-3

Things to Do

1. Request Japanese plasmid which overexpresses leucine in E. coli- DONE

2. Ask Professor Prather if she thinks that leucine is limiting in our reaction- DONE

3. Perform experiment that Reshma suggested (add .02 g/L of leucine to J45600 and J45900 cultures)

4. Resend request to Yale for aroF- aroG- aroH-, make sure it gets ordered today

5. Ask Endy if they think that it is a good idea to add new promoters/RBSs to precursor devices in parallel with obtaining overexpressing strains/what overall goals should be a priority

6. Run GC samples from yesterday's time course

7. Meet with Alex about GC (2 PM)

8. Print out GC procedure for isoamyl acetate for meeting- DONE