IGEM:MIT/2006/Notebook/2007-7-3

Things to Do

1. Request Japanese plasmid which overexpresses leucine in E. coli

2. Ask Professor Prather if she thinks that leucine is limiting in our reaction

3. Perform experiment that Reshma suggested (add .02 g/L of leucine to J45600 and J45900 cultures)

4. Resend request to Yale for aroF- aroG- aroH-, make sure it gets ordered today

5. Ask Knight/Endy/grad students if they think that it is a good idea to add new promoters/RBSs to precursor devices in parallel with obtaining overexpressing strains

6. Run GC samples from yesterday's time course