IGEM:MIT/2006/Notebook/2007-7-3
Things to Do
1. Request Japanese plasmid which overexpresses leucine in E. coli- DONE
2. Ask Professor Prather if she thinks that leucine is limiting in our reaction- DONE
3. Perform experiment that Reshma suggested (add .02 g/L of leucine to J45600 and J45900 cultures)
4. Resend request to Yale for aroF- aroG- aroH-, make sure it gets ordered today- WORKING ON IT
5. Ask Endy if they think that it is a good idea to add new promoters/RBSs to precursor devices in parallel with obtaining overexpressing strains/what overall goals should be a priority- DONE
6. Run GC samples from yesterday's time course- WORKING ON IT
7. Meet with Alex about GC (2 PM)- DONE (need to prepare isoamyl acetate in heptane and heptane extraction of LB media, Alex seemed optimistic)
8. Print out GC procedure for isoamyl acetate for meeting- DONE
Results from Time Courses
J45120 (Constitutive Promoter)
Time (Hrs)------------OD600-----------Methyl Salicylate (ppm)
3---------------------0.01------------NOT DETECTED
5---------------------0.21------------(-0.4)
7---------------------1.09------------
9--------------------2.5--------------
11--------------------2.5-------------
13--------------------2.8-------------
J45181 (Exponential-Phase Sensitive Promoter)
Time (Hrs)------------OD600-----------Methyl Salicylate (ppm)
3---------------------0.01------------NOT DETECTED
5---------------------0.27------------(0.1)
7---------------------1.15------------
9--------------------2.0--------------
11--------------------3.0-------------
13--------------------3.2-------------