IGEM:MIT/2006/Notebook/2007-8-13

Things to Do

1. Was going to do another time course of J45120 and J45181 but could only find 13 flasks. Decided to do growth curves for J45200 and J45250 with isoamyl alcohol added initially (started 3:13 AM)

2. Set up J45200 25-mL cultures to supplement with methyl salicylate later to develop extraction efficiencies (started 3:15 AM)

3. Put together package of "smelly strains" with J45120, J45200, isoamyl alcohol, and salicylic acid with instructions

4. Miniprep and submit sequencing of J45250 1AT3 before 10 AM- DONE

5. Reply to Lily about GC

6. Ask Alex about DB-17 column

7. Clean up lab area

8. Ask Drew 2 safety questions

9. GC extract J45200 cultures after adding varying amounts of methyl salicylate

OD600s

J45200

6 hrs- 0.18

7 hrs- 0.46

8 hrs-

9 hrs-

10 hrs-

11 hrs-

12 hrs-

13 hrs-

J45250

6 hrs- 0.30

7 hrs- 0.68

8 hrs-

9 hrs-

10 hrs-

11 hrs-

12 hrs-

13 hrs-