IGEM:MIT/2006/Notebook/2007-8-15: Difference between revisions
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| Line 21: | Line 21: | ||
Hour 10- 2.48 | Hour 10- 2.48 | ||
Hour 11- | Hour 11- 2.66 | ||
Hour 12- | Hour 12- 2.68 | ||
''J45181'' | ''J45181'' | ||
| Line 39: | Line 39: | ||
Hour 9- 2.28 | Hour 9- 2.28 | ||
Hour 10- | Hour 10- 2.70 | ||
Hour 11- | Hour 11- 2.60 | ||
Hour 12- | Hour 12- 2.62 | ||
| Line 58: | Line 58: | ||
Hour 10- 2.00 | Hour 10- 2.00 | ||
Hour 11- | Hour 11- 2.18 | ||
Hour 12- | Hour 12- 2.36 | ||
Hour 13- | Hour 13- 2.56 | ||
Hour 14- | Hour 14- 2.64 | ||
Hour 15- | Hour 15- 2.74 | ||
''J45181'' | ''J45181'' | ||
| Line 76: | Line 76: | ||
Hour 9- 1.62 | Hour 9- 1.62 | ||
Hour 10- | Hour 10- 1.96 | ||
Hour 11- | Hour 11- 2.26 | ||
Hour 12- | Hour 12- 2.30 | ||
Hour 13- | Hour 13- 2.48 | ||
Hour 14- | Hour 14- 2.48 | ||
Hour 15- | Hour 15- 2.60 | ||
=Other Things to Do= | |||
1. Ask Isadora to order more 125-mL flasks- DONE | |||
2. Ask Drew if it's okay to be late for lab meeting- DONE (OKAY) | |||
3. Bring GC column to Stephanopoulos lab- DONE | |||
4. Make GC reservations for tomorrow- DONE | |||
5. Set up fluorescent experiment following the procedure below- DONE | |||
*Grow up 5-mL B0015, R0040.E0840, J45995, and J45996 cultures for 17 hours- DONE | |||
*Dilute each culture 1:250 in a 25-mL culture in a 250-mL baffled flask- DONE | |||
*Grow these cultures for 4 hours at 220 RPM at 37C- DONE | |||
*Take OD600s of each culture: J45995- 1.28, J45996- 1.22, B0015- 0.88, R0040.E0840- 0.64- DONE | |||
*Dilute the cultures: 2.5 mL for J45995 (22.5 mL media), 2.62 mL for J45996 (22.4 mL media), 3.64 mL for B0015 (21.4 mL media), 5 mL for R0040.E0840 (20 mL media) (all in 250-mL baffled flasks)- DONE | |||
*Grow the cultures at 220 RPM for 50 mins- DONE | |||
*Remove 200 uL triplet samples from each culture to run on the plate reader- DONE | |||
Latest revision as of 14:58, 15 August 2007
J45120/J45181 Time Course
Used protocol in yesterday's notebook entry
OD600s before salicylic acid addition
J45120
Hour 4- 0.22
Hour 5- 0.36
Hour 6- 0.90
Hour 7- 1.50
Hour 8- 1.96
Hour 9- 2.30
Hour 10- 2.48
Hour 11- 2.66
Hour 12- 2.68
J45181
Hour 4- 0.16
Hour 5- 0.36
Hour 6- 0.72
Hour 7- 1.20
Hour 8- 1.76
Hour 9- 2.28
Hour 10- 2.70
Hour 11- 2.60
Hour 12- 2.62
OD600s after salicylic acid addition
J45120
Hour 7- 1.02
Hour 8- 1.36
Hour 9- 1.70
Hour 10- 2.00
Hour 11- 2.18
Hour 12- 2.36
Hour 13- 2.56
Hour 14- 2.64
Hour 15- 2.74
J45181
Hour 7- 0.80
Hour 8- 1.24
Hour 9- 1.62
Hour 10- 1.96
Hour 11- 2.26
Hour 12- 2.30
Hour 13- 2.48
Hour 14- 2.48
Hour 15- 2.60
Other Things to Do
1. Ask Isadora to order more 125-mL flasks- DONE
2. Ask Drew if it's okay to be late for lab meeting- DONE (OKAY)
3. Bring GC column to Stephanopoulos lab- DONE
4. Make GC reservations for tomorrow- DONE
5. Set up fluorescent experiment following the procedure below- DONE
- Grow up 5-mL B0015, R0040.E0840, J45995, and J45996 cultures for 17 hours- DONE
- Dilute each culture 1:250 in a 25-mL culture in a 250-mL baffled flask- DONE
- Grow these cultures for 4 hours at 220 RPM at 37C- DONE
- Take OD600s of each culture: J45995- 1.28, J45996- 1.22, B0015- 0.88, R0040.E0840- 0.64- DONE
- Dilute the cultures: 2.5 mL for J45995 (22.5 mL media), 2.62 mL for J45996 (22.4 mL media), 3.64 mL for B0015 (21.4 mL media), 5 mL for R0040.E0840 (20 mL media) (all in 250-mL baffled flasks)- DONE
- Grow the cultures at 220 RPM for 50 mins- DONE
- Remove 200 uL triplet samples from each culture to run on the plate reader- DONE
