IGEM:MIT/2006/Notebook/2007-8-15
J45120/J45181 Time Course
Used protocol in yesterday's notebook entry
OD600s before salicylic acid addition
J45120
Hour 4- 0.22
Hour 5- 0.36
Hour 6- 0.90
Hour 7- 1.50
Hour 8- 1.96
Hour 9- 2.30
Hour 10- 2.48
Hour 11- 2.66
Hour 12- 2.68
J45181
Hour 4- 0.16
Hour 5- 0.36
Hour 6- 0.72
Hour 7- 1.20
Hour 8- 1.76
Hour 9- 2.28
Hour 10- 2.70
Hour 11- 2.60
Hour 12- 2.62
OD600s after salicylic acid addition
J45120
Hour 7- 1.02
Hour 8- 1.36
Hour 9- 1.70
Hour 10- 2.00
Hour 11- 2.18
Hour 12- 2.36
Hour 13- 2.56
Hour 14-
Hour 15-
J45181
Hour 7- 0.80
Hour 8- 1.24
Hour 9- 1.62
Hour 10- 1.96
Hour 11- 2.26
Hour 12- 2.30
Hour 13-
Hour 14-
Hour 15-
Other Things to Do
1. Ask Isadora to order more 125-mL flasks- DONE
2. Ask Drew if it's okay to be late for lab meeting- DONE (OKAY)
3. Bring GC column to Stephanopoulos lab
4. Make GC reservations for tomorrow
5. Set up fluorescent experiment following the procedure below- IN PROGRESS
- Grow up 5-mL B0015, R0040.E0840, J45995, and J45996 cultures for 17 hours- DONE
- Dilute each culture 1:250 in a 25-mL culture in a 250-mL baffled flask- DONE
- Grow these cultures for 4 hours at 220 RPM at 37C
- Take OD600s of each culture
- Dilute the cultures
- Grow the cultures at 220 RPM for 50 mins
- Remove 200 uL triplet samples from each culture to run on the plate reader
