IGEM:MIT/2006/Notebook/2007-8-16: Difference between revisions
(New page: =Three things today= 1. Miniprep and sequence LC of J45250 1AT3- DONE (they said I'll most definitely get results by Monday morning, so I can hopefully start a banana time course; if not,...) |
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= | =Things today= | ||
1. Miniprep and sequence LC of J45250 1AT3- DONE (they said I'll most definitely get results by Monday morning, so I can hopefully start a banana time course; if not, I have the prepped DNA of the correct J45250 1AT3 whose LC I stupidly threw out, so I can transform that and start a new LC) | 1. Miniprep and sequence LC of J45250 1AT3- DONE (they said I'll most definitely get results by Monday morning, so I can hopefully start a banana time course; if not, I have the prepped DNA of the correct J45250 1AT3 whose LC I stupidly threw out, so I can transform that and start a new LC) | ||
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2. Data analysis of plate reader results- DONE (injector still broken; growth curves look like rainbows) | 2. Data analysis of plate reader results- DONE (injector still broken; growth curves look like rainbows) | ||
3. Run ALL 18 GC samples | 3. Run ALL 18 GC samples- DONE | ||
4. Make the following cultures to prepare for GC tomorrow: | |||
*25-mL J45200 w/ precursor | |||
*25-mL J45250 (1AT3) w/ precursor | |||
*25-mL J45200 w/o precursor (control) | |||
*25-mL J45600 | |||
*25-mL J45900 | |||
'''Calculation of Precursor''' | |||
We initially forgot to take into account isoamyl alcohol's density when deciding at which concentration to add isoamyl alcohol. | |||
We used 4.4 uL/10 mL because we assumed density to be 1 g/mL... Thus, .0044 g/.01 L*1 mol/88.2 g = 5 mM | |||
To make the final concentration be 5 mM, we actually want 4.4 uL/.809 (g/mL density)= 5.44 uL. | |||
=Results from GC= | |||
'''J45120''' | |||
4-7 Hours: 1.1 ppm | |||
5-8 Hours: 13.7 ppm | |||
6-9 Hours: 13.9 ppm | |||
7-10 Hours: 40.7 ppm | |||
8-11 Hours: 62.3 ppm | |||
9-12 Hours: 67.6 ppm | |||
10-13 Hours: 59.4 ppm | |||
11-14 Hours: 66.6 ppm | |||
12-15 Hours: 50.6 ppm | |||
'''J45181''' | |||
4-7 Hours: 7.9 ppm | |||
5-8 Hours: 14.4 ppm | |||
6-9 Hours: 27.3 ppm | |||
7-10 Hours: 59.0 ppm | |||
8-11 Hours: 80.6 ppm | |||
9-12 Hours: 80.1 ppm | |||
10-13 Hours: 63.4 ppm | |||
11-14 Hours: 50.2 ppm | |||
12-15 Hours: 37.7 ppm | |||
Latest revision as of 16:07, 16 August 2007
Things today
1. Miniprep and sequence LC of J45250 1AT3- DONE (they said I'll most definitely get results by Monday morning, so I can hopefully start a banana time course; if not, I have the prepped DNA of the correct J45250 1AT3 whose LC I stupidly threw out, so I can transform that and start a new LC)
2. Data analysis of plate reader results- DONE (injector still broken; growth curves look like rainbows)
3. Run ALL 18 GC samples- DONE
4. Make the following cultures to prepare for GC tomorrow:
- 25-mL J45200 w/ precursor
- 25-mL J45250 (1AT3) w/ precursor
- 25-mL J45200 w/o precursor (control)
- 25-mL J45600
- 25-mL J45900
Calculation of Precursor
We initially forgot to take into account isoamyl alcohol's density when deciding at which concentration to add isoamyl alcohol.
We used 4.4 uL/10 mL because we assumed density to be 1 g/mL... Thus, .0044 g/.01 L*1 mol/88.2 g = 5 mM
To make the final concentration be 5 mM, we actually want 4.4 uL/.809 (g/mL density)= 5.44 uL.
Results from GC
J45120
4-7 Hours: 1.1 ppm
5-8 Hours: 13.7 ppm
6-9 Hours: 13.9 ppm
7-10 Hours: 40.7 ppm
8-11 Hours: 62.3 ppm
9-12 Hours: 67.6 ppm
10-13 Hours: 59.4 ppm
11-14 Hours: 66.6 ppm
12-15 Hours: 50.6 ppm
J45181
4-7 Hours: 7.9 ppm
5-8 Hours: 14.4 ppm
6-9 Hours: 27.3 ppm
7-10 Hours: 59.0 ppm
8-11 Hours: 80.6 ppm
9-12 Hours: 80.1 ppm
10-13 Hours: 63.4 ppm
11-14 Hours: 50.2 ppm
12-15 Hours: 37.7 ppm
