IGEM:MIT/2006/Notebook/2007-8-17: Difference between revisions
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(New page: =Things to Do Today= 1. Make reservations for the plate reader (which is supposedly fixed)- DONE (5:00 PM tomorrow) 2. GC extract cultures from last night adding internal and external st...) |
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1. Make reservations for the plate reader (which is supposedly fixed)- DONE (5:00 PM tomorrow) | 1. Make reservations for the plate reader (which is supposedly fixed)- DONE (5:00 PM tomorrow) | ||
2. GC extract cultures from last night adding internal and external standards | 2. GC extract cultures from last night adding internal (hexyl acetate before extraction) and external standards (octyl acetate after extraction | ||
3. Make dilutions of isoamyl acetate in heptane plus | *We want 200 ppm | ||
3. Make dilutions of isoamyl acetate in heptane plus external standard (octyl acetate) | |||
4. Make LCs of J45995, J45996, B0015, and R0040.E0840 at the end of the day | 4. Make LCs of J45995, J45996, B0015, and R0040.E0840 at the end of the day | ||
Revision as of 07:57, 17 August 2007
Things to Do Today
1. Make reservations for the plate reader (which is supposedly fixed)- DONE (5:00 PM tomorrow)
2. GC extract cultures from last night adding internal (hexyl acetate before extraction) and external standards (octyl acetate after extraction
- We want 200 ppm
3. Make dilutions of isoamyl acetate in heptane plus external standard (octyl acetate)
4. Make LCs of J45995, J45996, B0015, and R0040.E0840 at the end of the day
5. Flesh out next step for wintergreen time courses with advisors
6. Grow up 250-mL culture of J45200 for comprehensive GC extraction experiment tomorrow
