IGEM:MIT/2006/Notebook/2007-8-17: Difference between revisions
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1. Make reservations for the plate reader (which is supposedly fixed)- DONE (5:00 PM tomorrow) | 1. Make reservations for the plate reader (which is supposedly fixed)- DONE (5:00 PM tomorrow) | ||
2. GC extract cultures from last night adding internal (hexyl acetate before extraction) and external standards (octyl acetate after extraction | 2. GC extract cultures from last night adding internal (hexyl acetate before extraction) and external standards (octyl acetate after extraction) for J45200 and J45250 (just want to see if we see a peak for J45600 and J45900) | ||
*We want 200 ppm | *We want 200 ppm internal and external standard. | ||
*For hexyl acetate, .87 g/mL/.0002 g/mL= 1:4350 dilution; in 25 mL, 25 mL/4350 = 5.75 uL; add 5.75 uL to cultures before extraction | |||
*For oxyl acetate, .856 g/mL/.0002 g/mL= 1:4280 dilution; in 1 mL, 1 mL/4280 = .2336 uL; Make a 1:10 dilution in heptane and add 2.336 uL to 1 mL sample | |||
3. Make dilutions of isoamyl acetate in heptane plus external standard (octyl acetate) | 3. Make dilutions of isoamyl acetate in heptane plus external standard (octyl acetate) |
Revision as of 08:17, 17 August 2007
Things to Do Today
1. Make reservations for the plate reader (which is supposedly fixed)- DONE (5:00 PM tomorrow)
2. GC extract cultures from last night adding internal (hexyl acetate before extraction) and external standards (octyl acetate after extraction) for J45200 and J45250 (just want to see if we see a peak for J45600 and J45900)
- We want 200 ppm internal and external standard.
- For hexyl acetate, .87 g/mL/.0002 g/mL= 1:4350 dilution; in 25 mL, 25 mL/4350 = 5.75 uL; add 5.75 uL to cultures before extraction
- For oxyl acetate, .856 g/mL/.0002 g/mL= 1:4280 dilution; in 1 mL, 1 mL/4280 = .2336 uL; Make a 1:10 dilution in heptane and add 2.336 uL to 1 mL sample
3. Make dilutions of isoamyl acetate in heptane plus external standard (octyl acetate)
4. Make LCs of J45995, J45996, B0015, and R0040.E0840 at the end of the day
5. Flesh out next step for wintergreen time courses with advisors
6. Grow up 250-mL culture of J45200 for comprehensive GC extraction experiment tomorrow