IGEM:MIT/2006/Notebook/2007-8-18: Difference between revisions
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1. Fluorescent experiment setup: | 1. Fluorescent experiment setup: | ||
* After 20 hours of growth, dilute 1:250 in 25-mL cultures in 250-mL flasks and shake for 4 hours at 220 RPM at 37C | * After 20 hours of growth, dilute 1:250 in 25-mL cultures in 250-mL flasks and shake for 4 hours at 220 RPM at 37C- DONE | ||
* Take OD600s of those 25-mL cultures | * Take OD600s of those 25-mL cultures- DONE | ||
*Dilute the cultures 1:25 in 25-mL culture in 250-mL flasks adjusting for OD600 and shake for 50 mins at 220 RPM at 37C | *Dilute the cultures 1:25 in 25-mL culture in 250-mL flasks adjusting for OD600 and shake for 50 mins at 220 RPM at 37C | ||
*Calculations: | |||
*Extract 200 uL from each culture three times and make triplicate cultures for each part | *Extract 200 uL from each culture three times and make triplicate cultures for each part | ||
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4. Smell J45250 1AT3 culture with added precursor- DONE (SMELLS!) | 4. Smell J45250 1AT3 culture with added precursor- DONE (SMELLS!) | ||
5. Make LC out of a colony from streaked out J45250 1AT3 plate | 5. Make LC out of a colony from streaked out J45250 1AT3 plate- DONE | ||
6. Contact Lily about specifics of GC run tomorrow- DONE | 6. Contact Lily about specifics of GC run tomorrow- DONE | ||
Revision as of 15:12, 18 August 2007
Things to Do Today
1. Fluorescent experiment setup:
- After 20 hours of growth, dilute 1:250 in 25-mL cultures in 250-mL flasks and shake for 4 hours at 220 RPM at 37C- DONE
- Take OD600s of those 25-mL cultures- DONE
- Dilute the cultures 1:25 in 25-mL culture in 250-mL flasks adjusting for OD600 and shake for 50 mins at 220 RPM at 37C
- Calculations:
- Extract 200 uL from each culture three times and make triplicate cultures for each part
2. Obtain B-PER for GC lysis experiment- CONTACTED TOM AND AUSTIN, SAID TO ASK SAUER LAB, ASKED SAUER LAB (THEY HAVE IT BUT ONLY AS PERSONAL STOCK), EMAILED BIOSTUFF
3. Start a time course for J45120 and J45181 late at night
4. Smell J45250 1AT3 culture with added precursor- DONE (SMELLS!)
5. Make LC out of a colony from streaked out J45250 1AT3 plate- DONE
6. Contact Lily about specifics of GC run tomorrow- DONE
