IGEM:MIT/2006/Notebook/2007-8-18: Difference between revisions
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1. Fluorescent experiment setup: | 1. Fluorescent experiment setup: | ||
* After 20 hours of growth, dilute 1:250 in 25-mL cultures in 250-mL flasks and shake for 4 hours at 220 RPM at 37C | * After 20 hours of growth, dilute 1:250 in 25-mL cultures in 250-mL flasks and shake for 4 hours at 220 RPM at 37C- DONE | ||
* Take OD600s of those 25-mL cultures | * Take OD600s of those 25-mL cultures- DONE | ||
*Dilute the cultures 1:25 in 25-mL culture in 250-mL flasks adjusting for OD600 and shake for 50 mins at 220 RPM at 37C | *Dilute the cultures 1:25 in 25-mL culture in 250-mL flasks adjusting for OD600 and shake for 50 mins at 220 RPM at 37C- DONE | ||
* | *Calculations: | ||
R0040.E0840- 0.42 OD600- 2.667 mL to 22.3 mL media | |||
3. Start a time course for J45120 and J45181 late at night | J45995- 1.02 OD600- 1.098 mL to 23.9 mL media | ||
J45996- 1.12 OD600- 1 mL to 24 mL media | |||
B0015- 0.48 OD600- 2.333 mL to 22.7 mL media | |||
*Extract 200 uL from each culture three times and make triplicate cultures for each part- DONE | |||
2. Obtain B-PER for GC lysis experiment- CONTACTED TOM AND AUSTIN, SAID TO ASK SAUER LAB, ASKED SAUER LAB (THEY HAVE IT BUT ONLY AS PERSONAL STOCK), EMAILED BIOSTUFF (NO RESPONSE) | |||
3. Start a time course for J45120 and J45181 late at night- CAN"T DO WITHOUT LYSIS SOLUTION | |||
4. Smell J45250 1AT3 culture with added precursor- DONE (SMELLS!) | 4. Smell J45250 1AT3 culture with added precursor- DONE (SMELLS!) | ||
5. Make LC out of a colony from streaked out J45250 1AT3 plate | 5. Make LC out of a colony from streaked out J45250 1AT3 plate- DONE | ||
6. Contact Lily about specifics of GC run tomorrow | 6. Contact Lily about specifics of GC run tomorrow- DONE | ||
Latest revision as of 16:30, 18 August 2007
Things to Do Today
1. Fluorescent experiment setup:
- After 20 hours of growth, dilute 1:250 in 25-mL cultures in 250-mL flasks and shake for 4 hours at 220 RPM at 37C- DONE
- Take OD600s of those 25-mL cultures- DONE
- Dilute the cultures 1:25 in 25-mL culture in 250-mL flasks adjusting for OD600 and shake for 50 mins at 220 RPM at 37C- DONE
- Calculations:
R0040.E0840- 0.42 OD600- 2.667 mL to 22.3 mL media
J45995- 1.02 OD600- 1.098 mL to 23.9 mL media
J45996- 1.12 OD600- 1 mL to 24 mL media
B0015- 0.48 OD600- 2.333 mL to 22.7 mL media
- Extract 200 uL from each culture three times and make triplicate cultures for each part- DONE
2. Obtain B-PER for GC lysis experiment- CONTACTED TOM AND AUSTIN, SAID TO ASK SAUER LAB, ASKED SAUER LAB (THEY HAVE IT BUT ONLY AS PERSONAL STOCK), EMAILED BIOSTUFF (NO RESPONSE)
3. Start a time course for J45120 and J45181 late at night- CAN"T DO WITHOUT LYSIS SOLUTION
4. Smell J45250 1AT3 culture with added precursor- DONE (SMELLS!)
5. Make LC out of a colony from streaked out J45250 1AT3 plate- DONE
6. Contact Lily about specifics of GC run tomorrow- DONE
