IGEM:MIT/2006/Notebook/2007-8-22: Difference between revisions
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1. Run GC samples of lysed cells- DONE (NOTHING SHOWED UP! NO!) | 1. Run GC samples of lysed cells- DONE (NOTHING SHOWED UP! NO!) | ||
2. 10:55- J45120 15 hour sample extraction OD=2.06, 11:15- J45181 15 hour sample extraction, 11:55- J45120 16 hour sample extraction, 12:15- J45181 16 hour sample extraction, 12:55- J45120 17 hour sample extraction, 1:15- J45181 17 hour sample extraction | 2. 10:55- J45120 15 hour sample extraction OD=2.06, 11:15- J45181 15 hour sample extraction OD=1.98, 11:55- J45120 16 hour sample extraction, 12:15- J45181 16 hour sample extraction, 12:55- J45120 17 hour sample extraction, 1:15- J45181 17 hour sample extraction | ||
3. Run samples above on GC | 3. Run samples above on GC | ||
Revision as of 08:20, 22 August 2007
Schedule
1. Run GC samples of lysed cells- DONE (NOTHING SHOWED UP! NO!)
2. 10:55- J45120 15 hour sample extraction OD=2.06, 11:15- J45181 15 hour sample extraction OD=1.98, 11:55- J45120 16 hour sample extraction, 12:15- J45181 16 hour sample extraction, 12:55- J45120 17 hour sample extraction, 1:15- J45181 17 hour sample extraction
3. Run samples above on GC
4. 3:15- Remove J45995, J45996, R0040.E0840, B0015 cultures and dilute them 1:250 at 220 RPM at 37C for 4 hours
5. GC extract banana cultures from last night (use 2 mL heptane and 80 mL culture for J45600 and J45900)
6. Prepare slides for lab meeting 5-7
7. 7:15- Dilute J45995, J45996, R0040.E0840, B0015 cultures and run them at 220 RPM at 37C for 50 mins
8. Extract 200 uL from each culture and run on the plate reader
