IGEM:MIT/2006/Notebook/2007-8-22: Difference between revisions
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1. Run GC samples of lysed cells- DONE (NOTHING SHOWED UP! NO!) | 1. Run GC samples of lysed cells- DONE (NOTHING SHOWED UP! NO!) | ||
2. 10:55- J45120 15 hour sample extraction OD=2.06, 11:15- J45181 15 hour sample extraction OD=1.98, 11:55- J45120 16 hour sample extraction OD=2.18, 12:15- J45181 16 hour sample extraction OD=1.94, 12:55- J45120 17 hour sample extraction, 1:15- J45181 17 hour sample extraction | 2. 10:55- J45120 15 hour sample extraction OD=2.06, 11:15- J45181 15 hour sample extraction OD=1.98, 11:55- J45120 16 hour sample extraction OD=2.18, 12:15- J45181 16 hour sample extraction OD=1.94, 12:55- J45120 17 hour sample extraction OD=2.12, 1:15- J45181 17 hour sample extraction | ||
NOTE- I think that growing up the cultures in 125-mL flasks from the start altered the growth curves | NOTE- I think that growing up the cultures in 125-mL flasks from the start altered the growth curves | ||
Revision as of 10:10, 22 August 2007
Schedule
1. Run GC samples of lysed cells- DONE (NOTHING SHOWED UP! NO!)
2. 10:55- J45120 15 hour sample extraction OD=2.06, 11:15- J45181 15 hour sample extraction OD=1.98, 11:55- J45120 16 hour sample extraction OD=2.18, 12:15- J45181 16 hour sample extraction OD=1.94, 12:55- J45120 17 hour sample extraction OD=2.12, 1:15- J45181 17 hour sample extraction
NOTE- I think that growing up the cultures in 125-mL flasks from the start altered the growth curves
3. Confirm flight arrangements for conference- DONE
4. Run samples above on GC
5. 3:15- Remove J45995, J45996, R0040.E0840, B0015 cultures and dilute them 1:250 at 220 RPM at 37C for 4 hours
6. GC extract banana cultures from last night (use 2 mL heptane and 80 mL culture for J45600 and J45900)
7. Prepare slides for lab meeting 5-7
8. 7:15- Dilute J45995, J45996, R0040.E0840, B0015 cultures and run them at 220 RPM at 37C for 50 mins
9. Extract 200 uL from each culture and run on the plate reader
10. Email Lily about GC- DONE
