IGEM:MIT/2006/Notebook/2007-8-7: Difference between revisions
| Line 1: | Line 1: | ||
=Things to Do= | =Things to Do= | ||
1. GC extract 25-mL cultures of the following that have been growing since 11 pm Sunday: | 1. GC extract 25-mL cultures of the following that have been growing since 11 pm Sunday- DONE: | ||
*J45700 EZ media -Vitamins + Fructose OD600=2.78 | *J45700 EZ media -Vitamins + Fructose OD600=2.78 | ||
| Line 11: | Line 11: | ||
*J45800 EZ media -Vitamins -TRP -TYR -PHE + Fructose OD600=1.62 | *J45800 EZ media -Vitamins -TRP -TYR -PHE + Fructose OD600=1.62 | ||
2. Run the four GC samples plus TOP10 cells | 2. Run the four GC samples plus TOP10 cells- RUNNING | ||
3. Run the protein GFP gel samples on protein gel | 3. Run the protein GFP gel samples on protein gel- DONE | ||
4. Talk with Barry/Jason about banana biosynthesis- DONE (suggested meeting with everyone there including Drew to establish whether phase-sensitive control should be focus or smell without precursor should be focus- I agree, will discuss with Drew) | 4. Talk with Barry/Jason about banana biosynthesis- DONE (suggested meeting with everyone there including Drew to establish whether phase-sensitive control should be focus or smell without precursor should be focus- I agree, will discuss with Drew) | ||
5. | 5. Smell J45250 + J45181 mix cultures and see if they smell- DONE (for both high copy: mint in exponential, banana in stationary; for high copy mint and mid copy banana: mint in exponential, slight mint and slight banana in stationary) | ||
6. | 6. Make fresh 25-mL J45250 + J45181 cultures (diluted 1:100) with control of TOP 10 cells all with precursors added and grow them up at 220 RPM- DONE (for some reason not growing fast (even for these cells), will let them grow over night) | ||
7. Make | 7. Make glycerols of J45250 + J45181 cultures from last night- DONE | ||
8. | 8. Grow up 8 mL TOP 10 to 17 mL EZ media with glycerol to GC extract as a control for today's GC experiment- DONE (grew up for ~80 mins., OD600=1.82) | ||
9. | 9. Ask Reshma if she wants to be around for the protein gel- DONE | ||
10. | 10. Make GC reservations- DONE | ||
11. Make | 11. Talk with Drew about setting up a meeting- DONE (2:15 tomorrow) | ||
12. Send out an email about meeting tomorrow- DONE | |||
13. Make an LC of IlvE- strain | |||
14. Check for restriction sites in tyrB gene cassette | |||
Revision as of 14:25, 7 August 2007
Things to Do
1. GC extract 25-mL cultures of the following that have been growing since 11 pm Sunday- DONE:
- J45700 EZ media -Vitamins + Fructose OD600=2.78
- J45700 EZ media -Vitamins -TRP -TYR -PHE + Fructose OD600=2.40
- J45800 EZ media -Vitamins + Fructose OD600=2.30
- J45800 EZ media -Vitamins -TRP -TYR -PHE + Fructose OD600=1.62
2. Run the four GC samples plus TOP10 cells- RUNNING
3. Run the protein GFP gel samples on protein gel- DONE
4. Talk with Barry/Jason about banana biosynthesis- DONE (suggested meeting with everyone there including Drew to establish whether phase-sensitive control should be focus or smell without precursor should be focus- I agree, will discuss with Drew)
5. Smell J45250 + J45181 mix cultures and see if they smell- DONE (for both high copy: mint in exponential, banana in stationary; for high copy mint and mid copy banana: mint in exponential, slight mint and slight banana in stationary)
6. Make fresh 25-mL J45250 + J45181 cultures (diluted 1:100) with control of TOP 10 cells all with precursors added and grow them up at 220 RPM- DONE (for some reason not growing fast (even for these cells), will let them grow over night)
7. Make glycerols of J45250 + J45181 cultures from last night- DONE
8. Grow up 8 mL TOP 10 to 17 mL EZ media with glycerol to GC extract as a control for today's GC experiment- DONE (grew up for ~80 mins., OD600=1.82)
9. Ask Reshma if she wants to be around for the protein gel- DONE
10. Make GC reservations- DONE
11. Talk with Drew about setting up a meeting- DONE (2:15 tomorrow)
12. Send out an email about meeting tomorrow- DONE
13. Make an LC of IlvE- strain
14. Check for restriction sites in tyrB gene cassette
