IGEM:MIT/2006/Notebook/2007-8-7
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Things to Do
1. GC extract 25-mL cultures of the following that have been growing since 11 pm Sunday:
- J45700 EZ media -Vitamins + Fructose
- J45700 EZ media -Vitamins -TRP -TYR -PHE + Fructose
- J45800 EZ media -Vitamins + Fructose
- J45800 EZ media -Vitamins -TRP -TYR -PHE + Fructose
2. Run the four GC samples
3. Run the protein GFP gel samples on protein gel
4. Talk with Barry/Jason about banana biosynthesis
5. Make up new 5X supplements with and without TRP, TYR, PHE
6. Smell J45250 + J45181 mix cultures and see if they smell- DONE (for both high copy: mint in exponential, banana in stationary; for high copy mint and mid copy banana: mint in exponential, slight mint and slight banana in stationary)
7. Make fresh 25-mL J45250 + J45181 cultures (diluted 1:100) with control of TOP 10 cells all with precursors added and grow them up at 220 RPM- DONE
8. Make glycerols of J45250 + J45181 cultures from last night- DONE