IGEM:MIT/2006/Notebook/2007-8-8: Difference between revisions
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6. Prepare for presentation at 2:00 | 6. Prepare for presentation at 2:00 | ||
[[Image:080807stapha.jpg]] | [[Image:080807stapha.jpg|thumb|500px|'''Stationary phase''']] | ||
[[Image:080807exppha.jpg|thumb|500px|'''Exponential phase''']] | |||
SCRAP EVERYTHING BIOSYNTHESIS, PHASE CONTROL FULL FORCE!!! | SCRAP EVERYTHING BIOSYNTHESIS, PHASE CONTROL FULL FORCE!!! | ||
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1. Grow up overnights of: | 1. Grow up overnights of: | ||
J45200 | J45200- Check | ||
J45250 | J45250- Check | ||
J45120- Check | J45120- Check | ||
| Line 33: | Line 34: | ||
2. Streak out: | 2. Streak out: | ||
J45100 | J45100- Make up AMP/TET plates tomorrow | ||
3. Locate Digests: | 3. Locate Digests: | ||
J45250 EP- Check | J45250 EP- Check (put on 1AT3) | ||
R0011 ES- Check | R0011 ES- Check (put on 1AK3 with J45119, put on 1AT3 with Q119B) | ||
Q119B XP- Check | Q119B XP- Check (put on 1AT3) | ||
J45119 XP- Check | J45119 XP- Check (put on 1AK3) | ||
1AT3 EP- Check | 1AT3 EP- Check | ||
4. Ask Jason/Austin how long a prep and/or digest is good for | 1AK3 EP- Check | ||
4. Ask Jason/Austin how long a prep and/or digest is good for- DONE (just try it) | |||
5. Fit 18 125-mL holders into shaker | 5. Fit 18 125-mL holders into shaker- DONE | ||
6. Secure 18 125-mL flasks- DONE | 6. Secure 18 125-mL flasks- DONE | ||
7. Pick up Meagan's J45119 streak | 7. Pick up Meagan's J45119 streak- got prep (don't worry about right now) | ||
Latest revision as of 14:02, 23 February 2008
Things to Do Today
1. Prepare protein gel, take a picture, and analyze
2. GC extract LB J45700 (OD600=0.70), J45800 (OD600=3.10)- DONE
3. Run LB J45700 and J45800 on the GC
4. Look up rare codon usage for the six different proteins
5. Look up biobrick sites in J45600/J45900
6. Prepare for presentation at 2:00
SCRAP EVERYTHING BIOSYNTHESIS, PHASE CONTROL FULL FORCE!!!
WHAT TO DO:
1. Grow up overnights of:
J45200- Check
J45250- Check
J45120- Check
J45181- Check
2. Streak out:
J45100- Make up AMP/TET plates tomorrow
3. Locate Digests:
J45250 EP- Check (put on 1AT3)
R0011 ES- Check (put on 1AK3 with J45119, put on 1AT3 with Q119B)
Q119B XP- Check (put on 1AT3)
J45119 XP- Check (put on 1AK3)
1AT3 EP- Check
1AK3 EP- Check
4. Ask Jason/Austin how long a prep and/or digest is good for- DONE (just try it)
5. Fit 18 125-mL holders into shaker- DONE
6. Secure 18 125-mL flasks- DONE
7. Pick up Meagan's J45119 streak- got prep (don't worry about right now)
