IGEM:MIT/2006/Notebook/2008-1-22: Difference between revisions
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GC extracts for J45250- 2:40, 3:40, 4:20 | GC extracts for J45250- 2:40, 3:40, 4:20 | ||
GC extract of J45200-PC, J45900 (OD600=2.26), and TOP10+PC (OD600=2.82)- Start at 11:40 | GC extract of J45200-PC (OD600=2.02), J45900 (OD600=2.26), and TOP10+PC (OD600=2.82)- Start at 11:40 | ||
''OD600s'' | ''OD600s'' | ||
| Line 33: | Line 33: | ||
5:00- 0.24 | 5:00- 0.24 | ||
5:40 | 5:40- 0.46 | ||
After IA Addition | After IA Addition | ||
| Line 39: | Line 39: | ||
'''J45200''' | '''J45200''' | ||
7:00 | 7:00- 0.12 | ||
8:00 | 8:00- 0.70 | ||
8:40 | 8:40- 1.12 | ||
'''J45250''' | '''J45250''' | ||
7:00 | 7:00- 0.20 | ||
8:00 | 8:00- 1.10 | ||
8:40 | 8:40- 1.28 | ||
=Set up cultures= | |||
1. 60 mL J45700- 250-mL baffled | |||
2. 60 mL J45120- 250-mL baffled | |||
3. 25 mL J45120+PC- 125-mL | |||
4. 25 mL TOP10+PC- 125-mL | |||
5. 115 mL J45200- 250-mL baffled | |||
6. 115 mL J45900- 25-mL baffled | |||
Latest revision as of 19:17, 22 January 2008
Mini Time Course
Time Zero for J45200- 7:20
Time Zero for J45250- 7:40
Pulse for J45200- 11:20, 12:20, 1:00
Pulse for J45250- 11:40, 12:40, 1:20
GC extracts for J45200- 2:20, 3:20, 4:00
GC extracts for J45250- 2:40, 3:40, 4:20
GC extract of J45200-PC (OD600=2.02), J45900 (OD600=2.26), and TOP10+PC (OD600=2.82)- Start at 11:40
OD600s
Before IA Addition
J45200
4:00- 0.04
5:00- 0.14
5:40- 0.32
J45250
4:00- 0.04
5:00- 0.24
5:40- 0.46
After IA Addition
J45200
7:00- 0.12
8:00- 0.70
8:40- 1.12
J45250
7:00- 0.20
8:00- 1.10
8:40- 1.28
Set up cultures
1. 60 mL J45700- 250-mL baffled
2. 60 mL J45120- 250-mL baffled
3. 25 mL J45120+PC- 125-mL
4. 25 mL TOP10+PC- 125-mL
5. 115 mL J45200- 250-mL baffled
6. 115 mL J45900- 25-mL baffled
