IGEM:MIT/2006/Notebook/2008-1-22

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(Mini Time Course)
(Mini Time Course)
Line 43: Line 43:
8:00- 0.70
8:00- 0.70
-
8:40
+
8:40- 1.12
'''J45250'''
'''J45250'''

Revision as of 17:08, 22 January 2008

Mini Time Course

Time Zero for J45200- 7:20

Time Zero for J45250- 7:40

Pulse for J45200- 11:20, 12:20, 1:00

Pulse for J45250- 11:40, 12:40, 1:20

GC extracts for J45200- 2:20, 3:20, 4:00

GC extracts for J45250- 2:40, 3:40, 4:20

GC extract of J45200-PC (OD600=2.02), J45900 (OD600=2.26), and TOP10+PC (OD600=2.82)- Start at 11:40

OD600s

Before IA Addition

J45200

4:00- 0.04

5:00- 0.14

5:40- 0.32

J45250

4:00- 0.04

5:00- 0.24

5:40- 0.46

After IA Addition

J45200

7:00- 0.12

8:00- 0.70

8:40- 1.12

J45250

7:00- 0.20

8:00- 1.10

8:40

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