IGEM:MIT/2006/Notebook/2008-2-10

General

• 1 g/mL = 1000000 ppm (from Stephen)
• 1 ppm = μg/g = μg/ml = ng/mg = pg/μg = 10^-6 from [1]
• molecular weight of isoamyl acetate = 130.19 from [2]
• The FW of an ion species is equal to its concentration in ppm at 10^-3M. Fluoride has a FW of 19, hence a 10^-3M concentration is equal to 19ppm, 1M is equal to 19,000 ppm and 1ppm is equal to 5.2 x 10-5M. from [3]

• 1 g/mL = 1000000000 ppb
• 1 ppb has 1 gram of substance for every billion grams of solution (http://www.ilpi.com/msds/ref/concentration.html)
• A solution with 1 ppb of solute has 1 microgram (10-6 g) of material per liter.

Isoamyl alcohol

• 13.6 μL isoamyl alcohol in 25 mL culture
• molecular weight of isoamyl alcohol = 88.15 g/mol from [4]
• specific gravity = 0.813 at 15°C relative to water at 4°C (Merck Index)
• 5 mM isoamyl alcohol

Salicylic acid

• 276 ppm salicylic acid
• molecular weight of salicylic acid = 138.123 g/mol
• 276 ppm = 1.998e-3 M = 2 mM salicylic acid

Odor thresholds

Also see http://www.leffingwell.com/odorthre.htm

Methyl salicylate

• molecular weight = 152.15 g/mol
• odor threshold = 0.1 ppm = 0.7 μM
• 40 ppb (http://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/jf00091a074) = 0.3 μM

Isoamyl acetate

• molecular weight = 130.18 g/mol
• odor threshold = 0.22 ppm = 1.7 μM
• 2 ppb http://www.leffingwell.com/odorthre.htm = 0.002 μM = 20 nM
• 30 μg/L (http://pubs.acs.org/cgi-bin/article.cgi/jafcau/1997/45/i08/pdf/jf970280a.pdf?isMac=595703) = 0.2 μM