IGEM:MIT/2006/Poster

General Design Notes and Subsection Division/Progress

1. BO = INFORMATION COMPILER/TEMPLATE DESIGNER
2. Top/Title:
   • we need to list undergrad+grad+faculty names: suggestion is to fade out the perfume bottle in the top right corner and to write names over top in black
3. Left 1/3:
   • left corner top: rename this section as MOTIVATIONS KB - DONE (=combo of what/why/applications). we can put a few simple bullets similar to what is on wiki "alternative" above
   • left side middle: change to KEY PART CONTRIBUTIONS KB -- so rather than listing every intermediate part/etc. in an overwhelming fashion, we can just show our 5 main enzyme genes plus osmY in a chart:
     • "biobrick name/function/source: species + reference #"
   • left side bottom: CHARACTERIZATION BC -- need to simplify our graphs. too many points/colors. we can either average our data or show one run w/o duplicates. also, the font on the key should be larger.
4. Center/Right 2/3:
   • add title: SYSTEM VV - DONE
     • center/top = the diagram we used in past presentation graphing our ideal goal of wintergreen and then banana smell. this takes care of a lot of words
     • right/top = black box picture. lets make the boxes all black instead of grayish. also, we need to draw an arrow connecting the enzyme to the line between boxes. we should get rid of the media box. also right out generator instead of GD. and we need to put the chemical output from the boxes (and mint + banana icon if possible)
     • center/right middle section: 4 device pictures. we should get rid of all the text below pictures for poster. edit out the trunc and full part on the osmY parts.
     • bottom center/right: get rid of registry snapshot and team photo. replace with:
       • FUTURE WORK KB - DONE
       • APPLICATIONS KB - DONE
       • ACKNOWLEDGEMENTS/REFERENCES KB - DONE

ACKNOWLEDGEMENTS (RS, KB)

1. see the acknowledgements page!

MOTIVATIONS (KB)

1. scented bacterium are cool
2. scents can be useful as natural reporters
3. the biosynthesis of volatile metabolites is a promising yet unexplored area of synthetic biology
4. nobody has ever attempted to build an autonomous cellular scent system
5. our engineered scent devices are modular and portable
6. we invision a diverse array of future work and applications

FUTURE WORK (KB)

1. boost production of precursor compounds to enhance smell
2. characterize scent compounds via Gas Chromatography (GC)
3. port the Banana Generating Device to S. Cerivisae, baker's yeast
4. port the Wintergreen Generating Device to Pseudomonas Fluorescens

APPLICATIONS (KB)

1. Improve the lab environment for microbiologists working with Escherichia coli
2. Port our system to bacterial species involved in bioremediation
3. Implement our system in bacteria responsible for bad human odor in the mouth, armpits and feet
4. Engineer new flavors and scents in bread and beer

KEY PART CONTRIBUTIONS (KB)

• Biobrick--Name-------Description---------------------------------------------------Source-------------References
• J45017----pchBA------Salicylic Acid synthesis-----------------------------------P. fluorescens---------(1)
• J45004----BSMT-------Converts Salicylic Acid -> Methyl Salicylate-----Petunia X hybrida-----(2)
• J45008----BAT2-------1st step in Isoamyl Acid synthesis--------------------S. cerivisiae------------(3)
• J45009----THI3------- 2nd step in Isoamyl Acid synthesis-------------------S. cerivisiae-------------(4)
• J45014----ATF1-------Converts Isoamyl Acid -> Isoamyl Acetate--------S. cerivisiae-------------(5)
• J45992----osmY-------Stationary Phase promoter-------------------------------E. coli---------------------(6)

REFERENCES

• anybody who wants to add a reference (ESPECIALLY FOR ABOVE CHART) can upload that info. here!