IGEM:MIT/2007/Heavy Metals

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(Cadmium)
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*Promoters
*Promoters
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Pubmed: Plasmid pI258 (from S.aureus) cadmium resistance (cadA) gene, complete cds [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=986930&dopt=GenBank&WebEnv=00h1mfSbNcrWnt_GeEAeAclSzZ4C1a1RZb7zt6pF-ZLWCiSSMjA-Di7PFSlt0aBL40bjiOJR7ssMw%40264002C567DCA7D0_0078SID&WebEnvRq=1]
<biblio>
<biblio>
#Brocklehurst03 pmid=12901859
#Brocklehurst03 pmid=12901859

Revision as of 11:19, 6 July 2007

Contents

Reviews

  • A Good Overview (though slightly back-dated): Quantitative assessment of worldwide contamination of air, water and soil by trace metals [1]
  • A summary of Pollutants from the EPA [2]
  1. Paul D, Pandey G, Pandey J, and Jain RK. . pmid:15734556. PubMed HubMed [Paul05]
  2. Valls M and de Lorenzo V. . pmid:12413663. PubMed HubMed [Valls02]
All Medline abstracts: PubMed HubMed


Cadmium

  • MCL(Maximum Containment Level): 5 ppb
  • MCLG(Maximum Containment Level Goal): 5 ppb
  • Known Carcinogen
  • Heavy Metal Limits
  1. Staessen JA, Lauwerys RR, Ide G, Roels HA, Vyncke G, and Amery A. . pmid:7911869. PubMed HubMed [Staessen94]
  2. Satarug S, Baker JR, Urbenjapol S, Haswell-Elkins M, Reilly PE, Williams DJ, and Moore MR. . pmid:12505433. PubMed HubMed [Satarug03]
All Medline abstracts: PubMed HubMed


  • Cadmium Contamination Sites
  1. Ishihara T, Kobayashi E, Okubo Y, Suwazono Y, Kido T, Nishijyo M, Nakagawa H, and Nogawa K. . pmid:11376862. PubMed HubMed [Ishihara01]
Plachimada, India: [3]
    • statistics on Pb, Cd, Cr levels in well water surrounding a Coca-Cola plant (2006)
    • pictures too :)


  • Most recent(June06) paper on e.coli bioaccumulation of Cd
  1. Deng X, Yi XE, and Liu G. . pmid:16890348. PubMed HubMed [Deng06]


  • Promoters

Pubmed: Plasmid pI258 (from S.aureus) cadmium resistance (cadA) gene, complete cds [4]

  1. Brocklehurst KR, Megit SJ, and Morby AP. . pmid:12901859. PubMed HubMed [Brocklehurst03]
  • Brocklehurst - contains sequence for ZntR/CadR and PzntA/PcadA
    • Semmie's article on CadC linked with GFP [5]

Mercury

  • MCL: 2 ppb
  • MCLG: 2 ppb
  • 20 ppb makes the fish mercury buildup significant


  • Polluted sites
    • Onondaga lake [6] [7] [8]
    • Salt Plains National Wildlife Refuge [9]


  • Detection (to assess our system):


  • Promoter
  1. Hamlett NV, Landale EC, Davis BH, and Summers AO. . pmid:1328156. PubMed HubMed [Hamlett92]
  2. Park SJ, Wireman J, and Summers AO. . pmid:1312997. PubMed HubMed [Park92]
  3. Condee CW and Summers AO. . pmid:1334070. PubMed HubMed [Condee92]
  4. Gambill BD and Summers AO. . pmid:4092936. PubMed HubMed [Gambill85]
  5. Hansen LH and Sørensen SJ. . pmid:11094290. PubMed HubMed [Hansen00]
All Medline abstracts: PubMed HubMed
  • Condee - Contains sequence for merO/P region
  • Hansen - Plasmid with Pmer, MerR, and reporter (lux, lac, or gfp) tested in e.coli

Current Industry Filtration Techniques

Mercury: approved by EPA - Coagulation/Filtration; Granular Activated Carbon; Lime softening; Reverse osmosis.

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