IGEM:MIT/2007/Heavy Metals - Revision history

http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&feed=atom&action=history IGEM:MIT/2007/Heavy Metals - Revision history 2013-05-21T22:59:08Z Revision history for this page on the wiki MediaWiki 1.13.2 http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=130002&oldid=prev Cookb at 03:04, 14 July 2007 2007-07-14T03:04:37Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 03:04, 14 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 60:</td> <td colspan="2" class="diff-lineno">Line 60:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Cobbett02 pmid=12221971</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Cobbett02 pmid=12221971</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Pazirandeh98 pmid=9758845</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Pazirandeh98 pmid=9758845</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#Chen98 pmid=9758654</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></biblio></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></biblio></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Cookb http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=129959&oldid=prev Cookb at 22:41, 13 July 2007 2007-07-13T22:41:31Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:41, 13 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 31:</td> <td colspan="2" class="diff-lineno">Line 31:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Seems that Mejare01 does a nice job of comparing the different Cd-binding methods which have been tried.  According to them, the methods which gave the biggest increase (>60-fold) in binding capacity were those that expressed entire MT proteins (or domains of them).  '''However, they did not consider the papers where PCs were used, which seem to have much higher binding capacities.'''  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Seems that Mejare01 does a nice job of comparing the different Cd-binding methods which have been tried.  According to them, the methods which gave the biggest increase (>60-fold) in binding capacity were those that expressed entire MT proteins (or domains of them).  '''However, they did not consider the papers where PCs were used, which seem to have much higher binding capacities.'''  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The Bae papers seem to indicate that the EC20 motif can bind both Cd and Hg (higher affinity for Hg), and the absolute accumulation is higher than for the MT-derived peptides.  Also, EC20 binding still works in the presence of up to 200mM salt and even EDTA.  Might be a good idea to ask them for their <del class="diffchange diffchange-inline">Lbb</del>-OmpA-EC20 expression construct!   </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The Bae papers seem to indicate that the EC20 motif can bind both Cd and Hg (higher affinity for Hg), and the absolute accumulation is higher than for the MT-derived peptides.  Also, EC20 binding still works in the presence of up to 200mM salt and even EDTA.  Might be a good idea to ask them for their <ins class="diffchange diffchange-inline">Lpp</ins>-OmpA-EC20 expression construct!   </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Cookb http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=129958&oldid=prev Cookb at 22:41, 13 July 2007 2007-07-13T22:41:11Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:41, 13 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 36:</td> <td colspan="2" class="diff-lineno">Line 36:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Binding Sequences To Try'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Binding Sequences To Try'''</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The full Pazirandeh98 Hg/Cd-specific binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The full Pazirandeh98 Hg/Cd-specific binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*From DeSilva02, the MerP-derived Hg-specific binding sequence (GMTCCAA) could be repeated 3x:  GMTCCAAGMTCCAAGMTCCAA</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For good non-specific binding, best bet seems to be the PC-derived EC20 sequence: (EC)20-Gly</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For good non-specific binding, best bet seems to be the PC-derived EC20 sequence: (EC)20-Gly</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*From DeSilva02, the MerP-derived Hg-specific binding sequence (GMTCCAA) could be repeated 3x:  GMTCCAAGMTCCAAGMTCCAA</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For Cd-specific binding, could later use the CadA-derived sequence (GFTCANC)  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For Cd-specific binding, could later use the CadA-derived sequence (GFTCANC)  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Cookb http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=129955&oldid=prev Cookb at 22:36, 13 July 2007 2007-07-13T22:36:26Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:36, 13 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 71:</td> <td colspan="2" class="diff-lineno">Line 71:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Contamination Levels/Limits===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Contamination Levels/Limits===</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*MCL(Maximum Containment Level): 5 ppb</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*MCL(Maximum Containment Level): 5 ppb <ins class="diffchange diffchange-inline"> (equal to about 45nM)</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MCLG(Maximum Containment Level Goal): 5 ppb</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MCLG(Maximum Containment Level Goal): 5 ppb</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><biblio></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><biblio></div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 98:</td> <td colspan="2" class="diff-lineno">Line 98:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Mercury==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Mercury==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Contamination Levels/Limits===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Contamination Levels/Limits===</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*MCL: 2 ppb</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*MCL: 2 ppb <ins class="diffchange diffchange-inline"> (equal to about 10 nM)</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MCLG: 2 ppb</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*MCLG: 2 ppb</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*20 ppb makes the fish mercury buildup significant</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*20 ppb makes the fish mercury buildup significant</div></td></tr>  </table>

Cookb http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=129953&oldid=prev Cookb at 22:32, 13 July 2007 2007-07-13T22:32:54Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:32, 13 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 29:</td> <td colspan="2" class="diff-lineno">Line 29:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The motif (CGCCG)3 was found to bind both cadmium and mercury (see Pazirandeh98)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The motif (CGCCG)3 was found to bind both cadmium and mercury (see Pazirandeh98)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Seems that Mejare01 does a nice job of comparing the different Cd-binding methods which have been tried.  According to them, the methods which gave the biggest increase (>60-fold) in binding capacity were those that expressed entire MT proteins (or domains of them).  </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Seems that Mejare01 does a nice job of comparing the different Cd-binding methods which have been tried.  According to them, the methods which gave the biggest increase (>60-fold) in binding capacity were those that expressed entire MT proteins (or domains of them). <ins class="diffchange diffchange-inline"> '''However, they did not consider the papers where PCs were used, which seem to have much higher binding capacities.''' </ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The Bae papers seem to indicate that the EC20 motif can bind both Cd and Hg (higher affinity for Hg), and the absolute accumulation is higher than for the MT-derived peptides.  Also, EC20 binding still works in the presence of up to 200mM salt and even EDTA.  Might be a good idea to ask them for their Lbb-OmpA-EC20 expression construct!  </ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2" class="diff-lineno">Line 35:</td> <td colspan="2" class="diff-lineno">Line 37:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The full Pazirandeh98 Hg/Cd-specific binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The full Pazirandeh98 Hg/Cd-specific binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*From DeSilva02, the MerP-derived Hg-specific binding sequence (GMTCCAA) could be repeated 3x:  GMTCCAAGMTCCAAGMTCCAA</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*From DeSilva02, the MerP-derived Hg-specific binding sequence (GMTCCAA) could be repeated 3x:  GMTCCAAGMTCCAAGMTCCAA</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*For good non-specific binding, best bet seems to be <del class="diffchange diffchange-inline">alpha domain of human MT</del>-<del class="diffchange diffchange-inline">II </del>(<del class="diffchange diffchange-inline">29aa</del>)<del class="diffchange diffchange-inline">.  Sequence is:  CCSCCPVGCAKCAQGCICKGASDKCSCCA</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*For good non-specific binding, best bet seems to be <ins class="diffchange diffchange-inline">the PC</ins>-<ins class="diffchange diffchange-inline">derived EC20 sequence: </ins>(<ins class="diffchange diffchange-inline">EC</ins>)<ins class="diffchange diffchange-inline">20-Gly</ins></div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*For Cd-specific binding, could use the CadA-derived sequence (GFTCANC)  </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*For Cd-specific binding, could <ins class="diffchange diffchange-inline">later </ins>use the CadA-derived sequence (GFTCANC)  </div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Cookb http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=129941&oldid=prev Cookb at 21:20, 13 July 2007 2007-07-13T21:20:55Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 21:20, 13 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 28:</td> <td colspan="2" class="diff-lineno">Line 28:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The MT-like motif CCAA was found to highly selective for Hg, while CAAC had broad specificity (see DeSilva02)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The MT-like motif CCAA was found to highly selective for Hg, while CAAC had broad specificity (see DeSilva02)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The motif (CGCCG)3 was found to bind both cadmium and mercury (see Pazirandeh98)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The motif (CGCCG)3 was found to bind both cadmium and mercury (see Pazirandeh98)</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Seems that Mejare01 does a nice job of comparing the different Cd-binding methods which have been tried.  According to them, the methods which gave the biggest increase (>60-fold) in binding capacity were those that expressed entire MT proteins (or domains of them). </ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2" class="diff-lineno">Line 36:</td> <td colspan="2" class="diff-lineno">Line 38:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For Cd-specific binding, could use the CadA-derived sequence (GFTCANC)  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For Cd-specific binding, could use the CadA-derived sequence (GFTCANC)  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Seems that Mejare01 does a nice job of comparing the different Cd-binding methods which have been tried.  According to them, the methods which gave the biggest increase (>60-fold) in binding capacity were those that expressed entire MT proteins (or domains of them). </del>  </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>   </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Cookb http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=129935&oldid=prev Cookb at 20:54, 13 July 2007 2007-07-13T20:54:26Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:54, 13 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 30:</td> <td colspan="2" class="diff-lineno">Line 30:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==</del>Binding Sequences To Try<del class="diffchange diffchange-inline">==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''</ins>Binding Sequences To Try<ins class="diffchange diffchange-inline">'''</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The full Pazirandeh98 Hg/Cd-specific binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The full Pazirandeh98 Hg/Cd-specific binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*From DeSilva02, the MerP-derived Hg-specific binding sequence (GMTCCAA) could be repeated 3x:  GMTCCAAGMTCCAAGMTCCAA</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*From DeSilva02, the MerP-derived Hg-specific binding sequence (GMTCCAA) could be repeated 3x:  GMTCCAAGMTCCAAGMTCCAA</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*For good non-specific binding, best bet seems to be alpha domain of <del class="diffchange diffchange-inline">Human </del>MT-II is:  CCSCCPVGCAKCAQGCICKGASDKCSCCA</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*For good non-specific binding, best bet seems to be alpha domain of <ins class="diffchange diffchange-inline">human </ins>MT-II <ins class="diffchange diffchange-inline">(29aa).  Sequence </ins>is:  CCSCCPVGCAKCAQGCICKGASDKCSCCA</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For Cd-specific binding, could use the CadA-derived sequence (GFTCANC)  </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*For Cd-specific binding, could use the CadA-derived sequence (GFTCANC)  </div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Cookb http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=129934&oldid=prev Cookb at 20:53, 13 July 2007 2007-07-13T20:53:18Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:53, 13 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 29:</td> <td colspan="2" class="diff-lineno">Line 29:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The motif (CGCCG)3 was found to bind both cadmium and mercury (see Pazirandeh98)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The motif (CGCCG)3 was found to bind both cadmium and mercury (see Pazirandeh98)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*The full Pazirandeh98 binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*From DeSilva02, the MerP-derived Hg binding (very specific) sequence (GMTCCAA) could be repeated 3x</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*For cadmium binding, could use the CadA-derived sequence (GFTCANC) </del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Alpha domain of Human MT-II is:  CCSCCPVGCAKCAQGCICKGASDKCSCCA</del></div></td><td colspan="2"> </td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Binding Sequences To Try==</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*The full Pazirandeh98 Hg/Cd-specific binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*From DeSilva02, the MerP-derived Hg-specific binding sequence (GMTCCAA) could be repeated 3x:  GMTCCAAGMTCCAAGMTCCAA</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*For good non-specific binding, best bet seems to be alpha domain of Human MT-II is:  CCSCCPVGCAKCAQGCICKGASDKCSCCA</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*For Cd-specific binding, could use the CadA-derived sequence (GFTCANC) </ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Seems that Mejare01 does a nice job of comparing the different Cd-binding methods which have been tried.  According to them, the methods which gave the biggest increase (>60-fold) in binding capacity were those that expressed entire MT proteins (or domains of them).   </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Seems that Mejare01 does a nice job of comparing the different Cd-binding methods which have been tried.  According to them, the methods which gave the biggest increase (>60-fold) in binding capacity were those that expressed entire MT proteins (or domains of them).   </div></td></tr>  </table>

Cookb http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=129933&oldid=prev Cookb at 20:50, 13 July 2007 2007-07-13T20:50:52Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:50, 13 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 30:</td> <td colspan="2" class="diff-lineno">Line 30:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The full Pazirandeh98 binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*The full Pazirandeh98 binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*From DeSilva02, the MerP-derived Hg binding (very specific) sequence (GMTCCAA) could be repeated 3x</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*For cadmium binding, could use the CadA-derived sequence (GFTCANC) </ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Alpha domain of Human MT-II is:  CCSCCPVGCAKCAQGCICKGASDKCSCCA</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Alpha domain of Human MT-II is:  CCSCCPVGCAKCAQGCICKGASDKCSCCA</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Cookb http://www.openwetware.org/index.php?title=IGEM:MIT/2007/Heavy_Metals&diff=129927&oldid=prev Cookb at 20:08, 13 July 2007 2007-07-13T20:08:57Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 20:08, 13 July 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 29:</td> <td colspan="2" class="diff-lineno">Line 29:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The motif (CGCCG)3 was found to bind both cadmium and mercury (see Pazirandeh98)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**The motif (CGCCG)3 was found to bind both cadmium and mercury (see Pazirandeh98)</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The full Pazirandeh98 binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*</ins>The full Pazirandeh98 binding sequence (CGCCG) is:  CGCCGKGHCGCCGKGHCGCCG</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Alpha domain of Human MT-II is:  CCSCCPVGCAKCAQGCICKGASDKCSCCA</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*</ins>Alpha domain of Human MT-II is:  CCSCCPVGCAKCAQGCICKGASDKCSCCA</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>  </table>

Cookb