IGEM:MIT/2007/Heavy Metals

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Contents

Reviews

  • A Good Overview (though slightly back-dated): Quantitative assessment of worldwide contamination of air, water and soil by trace metals [1]
  • A summary of Pollutants from the EPA [2]

Cadmium

  • MCL(Maximum Containment Level): 5 ppb
  • MCLG(Maximum Containment Level Goal): 5 ppb
  • Known Carcinogen


  • Cadmium Contamination Sites
  1. Ishihara T, Kobayashi E, Okubo Y, Suwazono Y, Kido T, Nishijyo M, Nakagawa H, and Nogawa K. . pmid:11376862. PubMed HubMed [Ishihara01]
Plachimada, India: [3]
    • statistics on Pb, Cd, Cr levels in well water surrounding a Coca-Cola plant (2006)
    • pictures too :)


  • Most recent(June06) paper on e.coli bioaccumulation of Cd
  1. Deng X, Yi XE, and Liu G. . pmid:16890348. PubMed HubMed [Deng06]


  • Heavy Metal Limits
  1. Staessen JA, Lauwerys RR, Ide G, Roels HA, Vyncke G, and Amery A. . pmid:7911869. PubMed HubMed [Staessen94]
  2. Satarug S, Baker JR, Urbenjapol S, Haswell-Elkins M, Reilly PE, Williams DJ, and Moore MR. . pmid:12505433. PubMed HubMed [Satarug03]
All Medline abstracts: PubMed HubMed


  • Promoters
  1. Brocklehurst KR, Megit SJ, and Morby AP. . pmid:12901859. PubMed HubMed [Brocklehurst03]
  • contains sequence for ZntR/CadR and PzntA/PcadA
    • Semmie's article on CadC linked with GFP [4]


Mercury

  • MCL: 2 ppb
  • MCLG: 2 ppb
  • 20 ppb makes the fish mercury buildup significant


  • Polluted sites
    • Onondaga lake [5] [6] [7]
    • Salt Plains National Wildlife Refuge [8]


  • Detection (to assess our system):


  • Promoter
  1. Hansen LH and Sørensen SJ. . pmid:11094290. PubMed HubMed [Hansen00]
  • Plasmid with Pmer, MerR, and reporter (lux, lac, or gfp) tested in e.coli


Current Industry Filtration Techniques

Mercury: approved by EPA - Coagulation/Filtration; Granular Activated Carbon; Lime softening; Reverse osmosis.

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