IGEM:MIT/2007/Heavy Metals

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< IGEM:MIT | 2007
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Contents

Reviews

  • A Good Overview (though slightly back-dated): Quantitative assessment of worldwide contamination of air, water and soil by trace metals [1]
  • A summary of Pollutants from the EPA [2]

Cadmium

  • MCL(Maximum Containment Level): 5 ppb
  • MCLG(Maximum Containment Level Goal): 5 ppb
  • Known Carcinogen


  • Cadmium Contamination Sites
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Plachimada, India: [3]
    • statistics on Pb, Cd, Cr levels in well water surrounding a Coca-Cola plant (2006)
    • pictures too :)


  • Most recent(June06) paper on e.coli bioaccumulation of Cd
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  1. Error fetching PMID 16890348: [Deng06]


  • Heavy Metal Limits
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  2. Error fetching PMID 12505433: [Satarug03]
All Medline abstracts: PubMed HubMed


  • Promoters
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  • contains sequence for ZntR/CadR and PzntA/PcadA
    • Semmie's article on CadC linked with GFP [4]


Mercury

  • MCL: 2 ppb
  • MCLG: 2 ppb
  • 20 ppb makes the fish mercury buildup significant


  • Polluted sites
    • Onondaga lake [5] [6] [7]
    • Salt Plains National Wildlife Refuge [8]


  • Detection (to assess our system):


  • Promoter
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  • Plasmid with Pmer, MerR, and reporter (lux, lac, or gfp) tested in e.coli


Current Industry Filtration Techniques

Mercury: approved by EPA - Coagulation/Filtration; Granular Activated Carbon; Lime softening; Reverse osmosis.

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