IGEM:MIT/2007/Heavy Metals

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< IGEM:MIT | 2007
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Contents

Reviews

  • Excellent Summary of Bacterial Metal Resistance (a must read)
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  1. Error fetching PMID 16133099: [Silver05]
  • A Good Overview on Pollution (though slightly back-dated): Quantitative assessment of worldwide contamination of air, water and soil by trace metals [1]
  • A summary of Pollutants from the EPA [2]
  • Review Papers on Bioremediation
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  1. Error fetching PMID 15734556: [Paul05]
  2. Error fetching PMID 12413663: [Valls02]
  3. Error fetching PMID 10702338: [Bruins00]
All Medline abstracts: PubMed HubMed
  • Paul - discusses advantages/disadvantages/problems of bioremediation
  • Bruins, Valls - discusses various kinds of bioremediation systems

Heavy Metal Binding Proteins/Peptides

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  1. Error fetching PMID 10698802: [Samuelson00]
  2. Error fetching PMID 9758845: [Biosorbants]
  3. Error fetching PMID 10049868: [Kotrba99]
  4. Error fetching PMID 11164556: [Mejare01]
  5. Error fetching PMID 11710062: [Lu01]
  6. Error fetching PMID 11042548: [Bae00]
All Medline abstracts: PubMed HubMed

Cadmium

Summary

  • Cd causes damage to cells primarily by the generation of reactive oxygen species (ROS), which causes single-strand DNA damage and disrupts the synthesis of nucleic acids and proteins. Cd is also an inhibitor of the DNA mismatch repair system…Results of this study confirmed that Cd toxicity caused profound changes in gene expression in which several stress response systems were induced simultaneously.
  • known carcinogen
  • Most recent(June06) paper on e.coli bioaccumulation of Cd
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  1. Error fetching PMID 16890348: [Deng06]

Contamination Levels/Limits

  • MCL(Maximum Containment Level): 5 ppb
  • MCLG(Maximum Containment Level Goal): 5 ppb
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  1. Error fetching PMID 7911869: [Staessen94]
  2. Error fetching PMID 12505433: [Satarug03]
All Medline abstracts: PubMed HubMed


Cadmium Contamination Sites

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Plachimada, India: [3]
    • statistics on Pb, Cd, Cr levels in well water surrounding a Coca-Cola plant (2006)
    • pictures too :)


Promoters

Pubmed: Plasmid pI258 (from S.aureus) cadmium resistance (cadA) gene, complete cds [4]

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  1. Error fetching PMID 12901859: [Brocklehurst03]
  • Brocklehurst - contains sequence for ZntR/CadR and PzntA/PcadA
    • Semmie's article on CadC linked with GFP [5]

Mercury

Contamination Levels/Limits

  • MCL: 2 ppb
  • MCLG: 2 ppb
  • 20 ppb makes the fish mercury buildup significant

Polluted sites

    • Onondaga lake [6] [7] [8]
    • Salt Plains National Wildlife Refuge [9]


Detection (to assess our system)


Promoter

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Error fetching PMID 11094290:
  1. Error fetching PMID 1328156: [Hamlett92]
  2. Error fetching PMID 1312997: [Park92]
  3. Error fetching PMID 1334070: [Condee92]
  4. Error fetching PMID 4092936: [Gambill85]
  5. Error fetching PMID 11094290: [Hansen00]
All Medline abstracts: PubMed HubMed
  • Condee - Contains sequence for merO/P region
  • Hansen - Plasmid with Pmer, MerR, and reporter (lux, lac, or gfp) tested in e.coli

Sponge

  • Accumulation of Hg ions in cell membrane [10]

Current Industry Filtration Techniques

Mercury: approved by EPA - Coagulation/Filtration; Granular Activated Carbon; Lime softening; Reverse osmosis.

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