IGEM:MIT/2007/Ideas: Difference between revisions
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'''M1. Bacteria with squid reflecting protein (reflectin)''' | '''M1. Bacteria with squid reflecting protein (reflectin)''' | ||
*comments: | *comments: | ||
**Brian:[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14716016&dopt=Abstract | **Brian: 6 family members, all highly homologous | ||
**Brian: biggest issue could be solubility problems (E. Coli) | |||
**Brian: try expression in different systems where folding more likely to be correct (yeast, streptomyces, etc) | |||
**Brian: only 1 major publication, so very little known about possible chaperones [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14716016&dopt=Abstract (see reference)] | |||
Revision as of 10:56, 24 April 2007
Ideas to chew on
Some ideas from 4/23/07 meeting
M1. Bacteria with squid reflecting protein (reflectin)
- comments:
- Brian: 6 family members, all highly homologous
- Brian: biggest issue could be solubility problems (E. Coli)
- Brian: try expression in different systems where folding more likely to be correct (yeast, streptomyces, etc)
- Brian: only 1 major publication, so very little known about possible chaperones (see reference)
M2. Self mini-prepping bacteria
- comments: once triggered, will lyse, express RNases, and precipitate proteins and genomic DNA
M3. Bacteria with limited lifetime (telomeres)
- comments:
- Brian: streptomyces bacteria have linear genome
- Brian: e. coli w/ linear genomes have been constructed (see reference)
M4. Bacteria with removed/non-functional DNA
- comments: "minicells" will grow for several weeks
M5. Incorporating biobrick parts into minicell
- comments: difficult to produce in large quatities
M6. Magnetic alignment of bacteria
- comments:
- Brian: surface display of peptide which binds magnetic nanoparticles
- Brian: can we control number of bound nanoparticles via concentration (i.e. one NP per bacteria)?
- Brian: feasibility: can we generate enough force and torque on NP to align bacteria (calculations)
M7. Bacteria that illuminate when dark
- comments:
M8. Bacteria which synthesize vitamins
- comments:
M9. Sensing pH
- comments:
- Brian: idea -- use anthocyanins as pH sensor (expressed in plants such as red cabbage)
- Brian: E. Coli have been metabolically engineered to produce anthocyanin (see reference)
M10. Bacteria with kill switch
- comments:
M11. Bacteria battle
- comments:
- Forrest: Austin mentioned during the 4/23/07 meeting that this could be done in 2-D (on a dish)
- Forrest: Environmental conditions/stimuli can skew the outcome (e.g. shinning light or lowering pH causes on colony to have advantage over another)
Random ideas from Superphage (Forrest)
F1. Engineering bacteria to operate in extreme environment (extremophiles)
- bacteria that die when not in artificially harsh environments (i.e. bacteria that 'escaped' from lab would not thrive)
http://environment.newscientist.com/article/dn11614-extremeliving-bacteria-has-genome-sequenced.html
- comments:
F2. High protein bacteria/fungus
- Easy to grow, and highly-nutritious
- To be made into bread spread for poor or disaster-striken communities
- comments:
F3. Blood clotting phage/bacteria
- function like Chitosan bandaids
http://en.wikipedia.org/wiki/Chitosan
- comments:
F4. Bacteria that process animal waste to recover nutrients
- Recover proteins and other substances from pool of farm animal waste (e.g. the edible stuff floats to the top) and add back to animal feed
- comments:
F5. Food spoilage detection
- Add non-harmful bacteria to milk, meat packaging, etc; these bacteria grow slightly more easily that the usual bacteria that make people sick, and are highly visible (e.g. bright purple) when they grow
- If consumer sees purple, if means that the food is possibly spoiled
- comments:
