IGEM:MIT/2007/Notebook/2007-6-12: Difference between revisions
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*Mini-prep plasmid DNA (EGFP and NNX) | *Mini-prep plasmid DNA (EGFP and NNX) | ||
==Nanodrop (~20ng/ul) Protocol== | ==Nanodrop (~20ng/ul) Protocol== | ||
#Start ND-1000 program | |||
#Select "nucleic acid" | |||
#2 µL water | |||
#Push OK | |||
#Wipe top and bottom | |||
#2 µL EB buffer (or water if used for elution) | |||
#Select "blank" | |||
#Wipe top and bottom | |||
#2 µl sample | |||
#Select "measure" | |||
#Record the content of DNA in the lower right hand corner in ng/µl | |||
*PCR amplification of EGFP | *PCR amplification of EGFP | ||
*Run agarose gel of PCR products | *Run agarose gel of PCR products | ||
Revision as of 11:32, 13 June 2007
Grad Advisors
- Morning: HD
- Afternoon: Eric
LAB WORK
- Mini-prep plasmid DNA (EGFP and NNX)
Nanodrop (~20ng/ul) Protocol
- Start ND-1000 program
- Select "nucleic acid"
- 2 µL water
- Push OK
- Wipe top and bottom
- 2 µL EB buffer (or water if used for elution)
- Select "blank"
- Wipe top and bottom
- 2 µl sample
- Select "measure"
- Record the content of DNA in the lower right hand corner in ng/µl
- PCR amplification of EGFP
- Run agarose gel of PCR products
- PCR purify
- Digest vector and PCR product
Things we need
- Medium latex gloves
- timers
- calculators
