IGEM:MIT/2007/Notebook/2007-6-12: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
mNo edit summary |
|||
Line 17: | Line 17: | ||
#Select "measure" | #Select "measure" | ||
#Record the content of DNA in the lower right hand corner in ng/µl | #Record the content of DNA in the lower right hand corner in ng/µl | ||
==PCR amplification of EGFP== | |||
*Run agarose gel of PCR products | *Run agarose gel of PCR products | ||
*PCR purify | *PCR purify |
Revision as of 11:34, 13 June 2007
Grad Advisors
- Morning: HD
- Afternoon: Eric
LAB WORK
- Mini-prep plasmid DNA (EGFP and NNX)
Nanodrop (~20ng/ul) Protocol
- Start ND-1000 program
- Select "nucleic acid"
- 2 µL water
- Push OK
- Wipe top and bottom
- 2 µL EB buffer (or water if used for elution)
- Select "blank"
- Wipe top and bottom
- 2 µl sample
- Select "measure"
- Record the content of DNA in the lower right hand corner in ng/µl
PCR amplification of EGFP
- Run agarose gel of PCR products
- PCR purify
- Digest vector and PCR product
Things we need
- Medium latex gloves
- timers
- calculators