IGEM:MIT/2007/Notebook/2007-6-12: Difference between revisions
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*PCR purify | *PCR purify | ||
*Digest vector and PCR product | *Digest vector and PCR product | ||
[[Media:iGEMGel.jpg| Gel Image]] | |||
==Things we need== | ==Things we need== | ||
Revision as of 09:06, 13 June 2007
Grad Advisors
- Morning: HD
- Afternoon: Eric
LAB WORK
- Mini-prep plasmid DNA (EGFP and NNX)
- Nanodrop (~20ng/ul)
- PCR amplification of EGFP
- Run agarose gel of PCR products
- PCR purify
- Digest vector and PCR product
Things we need
- Medium latex gloves
- timers
- calculators
