IGEM:MIT/2007/Notebook/2007-6-12

Grad Advisors

• Morning: HD
• Afternoon: Eric

LAB WORK

• Mini-prep plasmid DNA (EGFP and NNX)

Nanodrop (~20ng/ul) Protocol

1. Start ND-1000 program
2. Select "nucleic acid"
3. 2 µL water
4. Push OK
5. Wipe top and bottom
6. 2 µL EB buffer (or water if used for elution)
7. Select "blank"
8. Wipe top and bottom
9. 2 µl sample
10. Select "measure"
11. Record the content of DNA in the lower right hand corner in ng/µl

PCR amplification of EGFP

• Run agarose gel of PCR products
• PCR purify
• Digest vector and PCR product

Gel Image

Things we need

• Medium latex gloves
• timers
• calculators