IGEM:MIT/2007/Notebook/2007-6-29: Difference between revisions
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| Line 19: | Line 19: | ||
*mRFP Derivatives | *mRFP Derivatives | ||
**mRFP1 The original | **mRFP1 The original | ||
**tdTomato | **tdTomato [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=55420622] | ||
***Brightest | |||
***Best quantum Yield | |||
***Double the molecular weight though | |||
**mCherry [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=55420612] | |||
***Longest wavelengths | |||
***Highest photostability | |||
***Fastest maturation | |||
***Excellent pH resistance | |||
***Quantum efficiency is slightly lower (0.22 versus 0.25 for mRFP1) | |||
***Higher extinction coefficient (due to near-complete maturation) | |||
***Tolerance of N-terminal fusions and photostability make mRFP1 obsolete | |||
<biblio> | <biblio> | ||
#Shaner04 pmid=15558047 | #Shaner04 pmid=15558047 | ||
</biblio> | </biblio> | ||
Revision as of 11:34, 29 June 2007
Agenda for Friday, June 29
- Morning
- pBAD pickup
- Is OmpA crucial to bacterial cell survival?
- Finalize oligos (restriction sites)
- Research "red team" stuff -- ion exchange
- Brian -- stainless steel, nylon binding?
- Afternoon
- Look for metal inducible promoters (Mer) to fuse to GFP/something FP/whatever
- Order above oligo
- Find/finalize vector (pBAD33?)
- Have everything ordered by the end of today!!
- Question to think about: metal ion diffusion into the cell or binding at the surface?
Display Proteins
- mRFP Derivatives
- mRFP1 The original
- tdTomato [1]
- Brightest
- Best quantum Yield
- Double the molecular weight though
- mCherry [2]
- Longest wavelengths
- Highest photostability
- Fastest maturation
- Excellent pH resistance
- Quantum efficiency is slightly lower (0.22 versus 0.25 for mRFP1)
- Higher extinction coefficient (due to near-complete maturation)
- Tolerance of N-terminal fusions and photostability make mRFP1 obsolete
