IGEM:MIT/2007/Notebook/2007-7-10

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Agenda

  • Red Team - Meeting Friday 11am w/ Drew and Tom
  • Metal Contacts
  • email Meinke
  • email Cad grad (mark.ensor@uky.edu)
  • email/call pUT-Mer - Copenhagen
  • Send for sequencing: miniprep of F2620, E1010, B0034
  • design/order Mer Primers
  • design/order FhuA Primers
  • High Copy Plasmids
  • Transform MerPR+MerR plasmids
  • competent cells - Tom?
  • Obtaining untreated polystyrene for filter


Checked Transformation Plates

DH5a in Amp (100µl) - 14 colonies
DH5a in Amp (300µl) - 60
DH5a in Kan (100µl) - 4
DH5a in Kan (300µl) - 10

XL-1 in Amp (20µl) - 720 colonies
XL-1 in Amp (200µl) - 4800
XL-1 in Kan (20µl) - 220
XL-1 in Kan (300µl) - 800

Innoculated 4 Liquid Cultures of Transformation from XL-1 in Kan (20µl) Plate

  • Used 5ml of LB+Kan
  • Placed in 37C room at around 12pm

Sent out F2620, B0034, E1010 for Sequencing

  • Used samples #2 because those were also used for the digestion/ligation/3A
  • Form 7942
  • Used 3µl of each miniprepped DNA sample, 1µl of VF2/VR, and 8µl H2O (12µl total)
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