IGEM:MIT/2007/Notebook/2007-7-16: Difference between revisions
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(New page: =F2620 Expression Protocol= #Create Liquid Cultures in LB Media #Dilute Back 1:500 #Grow up for an hour #Spin Down and Resuspend in PBS #Add AHL) |
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#Spin Down and Resuspend in PBS | #Spin Down and Resuspend in PBS | ||
#Add AHL | #Add AHL | ||
#Use plate reader to test expression | |||
=Today's Agenda= | |||
*Redo Overgrown Liquid Cultures | |||
*Send for Sequencing | |||
*Look for AHL Induced Expression | |||
*Cut CPX and Backbone for ligation | |||
*Start designing high copy backbone | |||
*PCR off relevant portion of Mer operon from pto40 | |||
*Organize fridge | |||
Revision as of 07:10, 16 July 2007
F2620 Expression Protocol
- Create Liquid Cultures in LB Media
- Dilute Back 1:500
- Grow up for an hour
- Spin Down and Resuspend in PBS
- Add AHL
- Use plate reader to test expression
Today's Agenda
- Redo Overgrown Liquid Cultures
- Send for Sequencing
- Look for AHL Induced Expression
- Cut CPX and Backbone for ligation
- Start designing high copy backbone
- PCR off relevant portion of Mer operon from pto40
- Organize fridge
