IGEM:MIT/2007/Notebook/2007-7-16

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F2620 Expression Protocol

  1. Create Liquid Cultures in LB Media
  2. Dilute Back 1:500
  3. Grow up for an hour
  4. Spin Down and Resuspend in PBS
  5. Add AHL
  6. Use plate reader to test expression


Today's Agenda

  • Redo Overgrown Liquid Cultures
  • Send for Sequencing
  • Look for AHL Induced Expression
  • Cut CPX and Backbone for ligation
  • Start designing high copy backbone
  • PCR off relevant portion of Mer operon from pto40
  • Organize fridge
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