IGEM:MIT/2007/Notebook/2007-7-16

F2620 Expression Protocol

1. Create Liquid Cultures in LB Media
2. Dilute Back 1:500 in LB Media
3. Grow up for an hour in warm room shaker
4. Spin Down, Decant, and Resuspend in PBS
5. Add AHL
6. Use plate reader to test expression

Today's Agenda

• Redo Overgrown Liquid Cultures
• Send for Sequencing
• Look for AHL Induced Expression
• Cut CPX and Backbone for ligation
• Start designing high copy backbone
• PCR off relevant portion of Mer operon from pto40
• Organize fridge