IGEM:MIT/2007/Notebook/2007-7-17: Difference between revisions
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(New page: =Agenda= *Test Standard Assembly of pSB3k3 with F2620, B0034, E1010 using AHL **LC grown overnight) |
(→Agenda) |
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*Test Standard Assembly of pSB3k3 with F2620, B0034, E1010 using AHL | *Test Standard Assembly of pSB3k3 with F2620, B0034, E1010 using AHL | ||
**LC grown overnight | **LC grown overnight | ||
==Digestion of CPX for 3A== | |||
<pre> | |||
1µl CPX (diluted to 1µg/µl) | |||
0.5µl BSA | |||
5µl NEB3 Buffer | |||
1µl XbaI | |||
1µl PstI | |||
41.5µl H2O | |||
------------------ | |||
50µl Total | |||
</pre> | |||
==Digestion of (F2620 and B0034) for 3A== | |||
<pre> | |||
20µl (F2620 and B0034) @ 35ng/µl | |||
0.5µl BSA | |||
5µl NEB2 Buffer | |||
1µl EcoRI | |||
1µl SpeI | |||
22.5µl H2O | |||
------------------ | |||
50µl Total | |||
</pre> | |||
*Digested pSB1AT3: 15µl @ 125 ng/µl |
Revision as of 14:25, 16 July 2007
Agenda
- Test Standard Assembly of pSB3k3 with F2620, B0034, E1010 using AHL
- LC grown overnight
Digestion of CPX for 3A
1µl CPX (diluted to 1µg/µl) 0.5µl BSA 5µl NEB3 Buffer 1µl XbaI 1µl PstI 41.5µl H2O ------------------ 50µl Total
Digestion of (F2620 and B0034) for 3A
20µl (F2620 and B0034) @ 35ng/µl 0.5µl BSA 5µl NEB2 Buffer 1µl EcoRI 1µl SpeI 22.5µl H2O ------------------ 50µl Total
- Digested pSB1AT3: 15µl @ 125 ng/µl