IGEM:MIT/2007/Notebook/2007-7-17

Agenda

• Test Standard Assembly of pSB3k3 with F2620, B0034, E1010 using AHL
  • LC grown overnight

Digestion of CPX for 3A

1µl       CPX (diluted to 1µg/µl)
0.5µl     BSA
5µl       NEB3 Buffer
1µl       XbaI
1µl       PstI
41.5µl    H2O
------------------
50µl      Total

Digestion of (F2620 and B0034) for 3A

20µl      (F2620 and B0034) @ 35ng/µl
0.5µl     BSA
5µl       NEB2 Buffer
1µl       EcoRI
1µl       SpeI
22.5µl    H2O
------------------
50µl      Total

• Digested pSB1AT3: 15µl @ 125 ng/µl