IGEM:MIT/2007/Notebook/2007-7-18: Difference between revisions
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(New page: ==Agenda== #Transform tk's competent cells with yesterday's 3A assembly results (F2620+B0034 and CPX insert into vector pSB1AC3) #Make LB+Cm and LB+Cm+Kan plates #Meeting with TK and Drew ...) |
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**1 negative control (pSB1AC3 digested with EcoRI and PstI) | **1 negative control (pSB1AC3 digested with EcoRI and PstI) | ||
*Followed our Transformation protocol (tk's modified protocol) | *Followed our Transformation protocol (tk's modified protocol) | ||
*Transformations were put into 37C room at 11:30 AM and incubated for 2 hrs | |||
===Poured Plates, again=== | ===Poured Plates, again=== |
Revision as of 09:35, 18 July 2007
Agenda
- Transform tk's competent cells with yesterday's 3A assembly results (F2620+B0034 and CPX insert into vector pSB1AC3)
- Make LB+Cm and LB+Cm+Kan plates
- Meeting with TK and Drew
Transformed tk's competent cells
- Aliquoted one of the original vials into eight eppendorfs, 100µL each.
- Did 3 sets of transformations:
- 2 of yesterday's 3A assembly product (plasmid contains: F2620+B0034+CPX in pSB1AC3 backbone)
- 1 negative control (pSB1AC3 digested with EcoRI and PstI)
- Followed our Transformation protocol (tk's modified protocol)
- Transformations were put into 37C room at 11:30 AM and incubated for 2 hrs
Poured Plates, again
- Used stringent working concentrations:
- 10µg of Kanamycin per mL of LB
- 25µg of Chloramphenicol per mL of LB
- Poured 5 LB+Cm+Kan plates
- Poured 29 LB+Cm plates
- Cm plates have green labels